We narrowed to 530 results for: abo.2

...two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a ...format. Figure 6: Add sequence, maps, and files. Step 2: Gene and Insert *Note: This page will not appear ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

...responsible for that differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

...retransduce a model organism such as mice, e.g. first at 2 months, then at 4 months? Or do antibodies become ...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses.  As always...

...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

... tabular HTML formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow...Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...

...cellular physiology.  https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020. “A glance at genome editing with...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...retroviral library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...

... 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK...within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your cells divide...

...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

...the details and learn more about colony PCR in this blog post.   Figure 2: The steps of colony PCR...often relies on antibiotic resistance. But what about identifying clones that contain your desired insert... restriction digest. The key is to be strategic about your enzyme choices. You’ll want to pick enzymes...Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning a gene into your ... and tips Resources on Addgene.org Learn more about Sanger sequencing Read the Molecular Biology Reference...

...JS, Legube G, Miller KM Genes Dev. 2015 Jan 15;29(2):197-211. DNA Cross-Bridging Shapes a Single Nucleus...Leah Bury of Microscopic Art. I started thinking about this problem when I recently attended the 2018 Chromosome...lot of scientists who not only remember to think about DNA in this context, but also specifically study...plasmids containing these genes. As with the PIs above, many of these genes were discussed at the 2018 ...chromatin This is not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest...

...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access..., social media, coffee chats with potential collaborators, blogging and more. These each have different...key routes for researchers to share information about the emerging COVID-19 pandemic quickly and openly...awareness of the benefits of preprints. Some worry about faulty work being posted as preprints, assuming ...submission. Preprints at Addgene Preprints are all about sharing scientific knowledge faster and more openly...

...state-of-the-art methods of sex and gender analysis and 2) provides case studies with concrete examples of how.... Gendered Innovations is an international, collaborative project—funded by the European Commission, the...research.  In addition to the studies referenced above, another excellent resource for research design ... whole human population. To learn a little more about where differences in effects on the sexes come from...cost. Especially in animal research, they worry about needing more study subjects and control groups. ...

...glycerol stock to each of 2 96-well microplates (identical copies, see image above). We then select forward...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...bp of clean sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature ...specifically to target this region, as outlined above. Avoid primers that bind within or must read through...

...IgM, IgD, and IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the...blog Learn about the advantages and disadvantages of using monoclonal antibodies Read about other techniques... subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101: What is an...

...available in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...aren’t reflected in the common components outlined above for gamma-retroviruses. While not required for lentiviral...and helper plasmids for all the viruses discussed above (gamma-retrovirus, lentivirus, adenovirus, and adeno-associated.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ...

...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...