We narrowed to 533 results for: erf

1. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism of...

2. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...modification(s) Congratulations! You’ve picked your perfect guide; now check out our blog posts on choosing...

3. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...exciting time to be a scientist looking for that perfect fit!  References and Resources Additional resources...

4. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute selectivity...

5. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...BioRender.com. With this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal...

6. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...

7. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...exciting applications, this system is an incredibly powerful tool for cloning complicated constructs in a single...

8. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...the selection marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing...

9. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...

10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...SplitSet™. With these tools, NEB successfully performed a 35-fragment Golden Gate reaction, using four-base...

11. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...Regardless of which ligand you choose, there is no “perfect” drug that only binds to one receptor, so it is...

12. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...antigen in a shorter period of time and would likely perform better for probing poorly expressed targets. Similarly...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ... study the mechanisms of infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab....

16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas...

17. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...the experiment. Handling can however negatively interfere with certain tests and is not always recommended...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...your parental mice to generate F1 Once you’ve performed your first cross, congratulations! You’ve generated...

20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...researchers with useful and adaptable tools to perform basic and preclinical research. For scientists ...