We narrowed to 415 results for: genome

1. Plasmids 101: Restriction Cloning

   Type

   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...chemicals to precipitate out the high molecular weight genomic DNA; filter the remaining plasmid DNA through a...

2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...example is our series of blog posts on the defunct genome editing technology NgAgo). In addition, there are...

4. Exploring Applications of the Bioluminescent HiBiT Tag

   Type

   Blog Post

   Published

   Oct. 2, 2025, 1:15 p.m.

   ... Tomida, S., Toyooka, S., & Otsuka, M. (2025). Genomic Differences and Distinct TP53 Mutation Site-Linked...

5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ..., we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional transgene that ...

6. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ...controlled through titer (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume....

7. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...scientist for a 6 month project to validate some genomic changes, so this arrangement was ideal for everyone...

8. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...sequence of interest are annealed to target DNA (genomic DNA or a PCR amplified fragment) and treated with...

9. Selecting Your Plasmid Purification Kit

   Type

   Blog Post

   Published

   May 27, 2025, 1:15 p.m.

   ...any step of the process and include things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins...

10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...aspects and recent applications." Current Chemical Genomics and Translational Medicine 3.1 (2009). PubMed ...

11. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    ..., & Chew, W. L. (2021). Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision...

12. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    Published

    Oct. 12, 2023, 1:15 p.m.

    ...Chen et al., 2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid... screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 and thus produces...

13. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...introduce genetic material into neuronal cells. The AAV genome is a single-stranded DNA molecule and AAV vectors...

14. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...Ronda et al., 2019). They called their method Metagenomic Alteration of Gut microbiome by In situ Conjugation... PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in...

15. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...Erb et al., Huang et al.), transcriptional and epigenomic profiling studies (Nabet et al., Erb et al., ...