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Showing: 401 - 420 of 678 results

  1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...lightbulb or exchanging excitation filters). This avoids substantial changes in excitation power and will...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    .... Cells are cultured with isotope-labeled amino acids for several hours to several days, followed by a... a short period of growth with unlabeled amino acids. This washout period removes radio-labeled short-... Resources on Addgene.org Fluorescent Protein Plasmids and Resources Fluorescent Protein Guide: Biosensors...

  4. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...stringent wash conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many...polyhistidine tag is a string of histidine amino acids on the N- or C- terminus of a protein. Neither the...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  6. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...problematic because they require radiolabeled amino acids. The use of radioactive materials is highly regulated...

  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...Addgene.org Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about...They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment. This ...

  8. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...create it. But while antibodies, as the OG (as the kids say) affinity reagents deserved to be celebrated...of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be used ...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...

  10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...ligation products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

  11. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance...of the retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in ...blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral...

  12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ... using an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/...Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins...Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...at a round-up of some new and popular split-FP plasmids from our catalog to help you find the right split-FP...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...from researchers, and the vast majority of our plasmids and pooled libraries were originally described...links to later papers that successfully used said plasmids. We provide these references as our way of saying...Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help with the Addgene...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... genes are supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and...carrying your gene of interest, and the two helper plasmids, commonly called “triple plasmid transfection”...genome-containing (full) and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible...

  18. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...fidelity and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central...

  19. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...sequencing on select regions of all distributed plasmids as part of quality control, but does not perform...complexes or with guide RNA- and Cas9-expression plasmids. Cas9 guide RNA delivery does however rely on ...happy genome editing! Check Out Other Argonaute Plasmids from the Doudna Lab Many thanks to our Guest ...

  20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ..."Addgene is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks...engineering an entire cell. Typically when people use plasmids, they are trying to do one thing at a time. What... tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic...
Showing: 401 - 420 of 678 results