We narrowed to 462 results for: invs
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TypeBlog PostPublishedFeb. 6, 2020, 3 p.m.... wanted to learn more, even those not directly involved in the project. Once we gauged interest from ...
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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m.... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m.... for working with lots of students, managing inventories in the lab, or organizing a research project,... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m.... that even though these early steps don’t even involve antibodies, they are still critical to the outcome... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....reviewing this post! Dr. Ben-Simon is an assistant investigator at the Allen Institute in the department of ... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....of the developers of the MoClo system, are an invaluable resource for learning about MoClo in an in-depth... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....This process builds on our former approach and involves reading primary literature, soliciting information... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....in lab to go to with questions and concerns is invaluable. With a little thought you can build meaningful... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....Bourdeau, R. W. et al. Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts.... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...