We narrowed to 465 results for: invs

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...Chen et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-...

3. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...easily accessible organ that can be monitored non-invasively over time. Importantly, it is an immune privileged...

4. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ... for working with lots of students, managing inventories in the lab, or organizing a research project,...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ... to be successful. A BiFC experiment generally involves three steps: fusion protein construction, cellular...

6. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...

7. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ... wanted to learn more, even those not directly involved in the project.  Once we gauged interest from ...

8. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ...interactive. If you can think of a good analogy that involves student participation, make it happen! How should...

9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...kanamycin resistance gene. Every step along the way involves good aseptic technique and controls to ensure ...

11. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...something will go awry. Don’t plan something that will involve a huge group getting from point A to B to C to ...

12. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...family. #7 Thou shalt create community to help Involve family. If you don’t have local family to help,...

13. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...programmable gene insertion with PASTE. The PASTEsystem involves insertion of landing sites via Cas9-directed reverse...

14. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

15. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...ChIP) technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP...

16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...This process builds on our former approach and involves reading primary literature, soliciting information...

17. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...

18. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type

    Blog Post

    Published

    Sept. 9, 2025, 1:15 p.m.

    ...reviewing this post! Dr. Ben-Simon is an assistant investigator at the Allen Institute in the department of ...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...of the developers of the MoClo system, are an invaluable resource for learning about MoClo in an in-depth...

20. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...sense once you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted...