We narrowed to 486 results for: mme

1. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...manually in water baths but is now easily done in a programmed thermocycler. For more details on this procedure...

2. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...questions during the talk, jot them down for the Q&A immediately after. For one conference I went to, I made it...

3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...let us know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved...

4. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...family-run business – 3D Molecular Designs – to commercialize some of the best instructional materials that...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...do the same for you. Send us your questions or comments to [email protected]. We're eager to ...

6. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem...

7. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...

8. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...making targeted modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. ...

9. Experimenting with New Careers while in Grad School

   Type

   Blog Post

   Published

   Feb. 25, 2016, 3:30 p.m.

   ...organization. My short experience with my blog helped immensely when CINSO started brainstorming the resources...

10. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...compared to the rest of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence...

11. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ... a backbone as a single unit. Although it is recommended to use the 2A peptides instead of an IRES when...

12. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...function of the FP-fusion protein. Therefore, it is recommended to use monomeric FPs (usually denoted by a “m...

13. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...involving sigmoidal curves, manufacturers often recommend using advanced graphing software designed to plot...

14. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...potential instabilities in the China data, so we recommend interpreting the model results for China with ...

17. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... humans (and no vaccines are available on the commercial market). Although host-virus interactions likely...

18. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter...

19. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    Published

    July 9, 2019, 12:52 p.m.

    ... to answer some of their great questions, was immensely gratifying. I came out of the experience with ...

20. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...