We narrowed to 683 results for: mos

... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...

...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...

...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

...common issues follow. It's a matter of concentration Most FPs in nature are prone to dimerization (i.e. EGFP... FPs such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs or FPs from different...

...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

... Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses. I could...inspiration for our updated mascot, it’s also a convenient most requested filter. Applying a second filter, this...

...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

...’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company... atmosphere...

...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

...every biologist, academic, and researcher. Among the most well-known CRISPR-associated nucleases are Cas9 ...just 408 amino acids long — making it one of the most compact genome editors to date. We have developed...

...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...

...communicate your story with only the most salient details. Make the most of your limited speaking time. Beyond...