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We narrowed to 662 results for: mpo

Showing: 401 - 420 of 662 results

  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...After a run on our Illumina MiSeq, the data is imported into our assembly pipeline that runs with Seqera...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...

  6. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology, or virology...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...subcellular environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  9. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  10. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

  14. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...hands, these combined pitfalls made it virtually impossible to find the 0.2-0.5% correctly edited alleles...to direct the zygosity of the cells. This was important for us because the Alzheimer mutations we study...

  15. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...

  17. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...

  18. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good...
Showing: 401 - 420 of 662 results