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  1. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

  4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...hands, these combined pitfalls made it virtually impossible to find the 0.2-0.5% correctly edited alleles...to direct the zygosity of the cells. This was important for us because the Alzheimer mutations we study...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...

  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...(FLuc) and Renilla (RLuc) luciferase. This is important not so much for how bright you can make a reaction...fusion protein called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good...

  11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...through click chemistry. Although these multi-component systems can be complicated to implement, they ...Ulrichs, H., Gaska, I., & Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by ...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective... system for direct identification of allelic composition of mutated DNA in a mixed population of excess...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cell’s size, granularity, and target protein composition. It is used for a variety of applications such...electronics systems, FACS instruments have specialized components that can divert a subset of cells. During FACS...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...excitation and emission wavelengths, it is also important to know the brightness of individual fluorophores...antibody-conjugated labeling. Even though the two compounds are completely different in nature (eGFP being...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...fragments, allowing ordered assembly of multiple components into a destination vector. The first step in ...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...

  20. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...
Showing: 401 - 420 of 650 results