We narrowed to 662 results for: mpo

...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...After a run on our Illumina MiSeq, the data is imported into our assembly pipeline that runs with Seqera...

... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...

...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...

...timetable for the Singletons in their bins.  The next component of Binning Singletons is when we bring all the...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through meetings...

...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...

...Collection aka ATCC where I work. It is virtually impossible to work in cell culture, microbiology, or virology...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...subcellular environments, including the organelles that compose the secretory pathway, endocytic vesicles, mitochondria...

... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

...hands, these combined pitfalls made it virtually impossible to find the 0.2-0.5% correctly edited alleles...to direct the zygosity of the cells. This was important for us because the Alzheimer mutations we study...

...plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling...sensors Whatever your analyte of interest, it’s important to choose a biosensor that has a binding affinity...

...should be supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in...Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat...

...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...Ashley Waldron The mammalian nervous system is composed of myriad cell types and subtypes, and a familiar...

...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

...generations of scFvs against two antigens that are impossible to generate scFvs against in vivo: antigens found...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...

...Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation...21408-21413. PUF Assembly Kit PUF proteins are composed of repeat segments that can be engineered to bind...

...which could potentially truncate the template). Importantly, you also need a DNA polymerase that produces...resulting construct. In cases where the functionally important regions span several kb (or are GC-rich and therefore...

...Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how a virus ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...

...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good...