Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 745 results for: nes

Showing: 401 - 420 of 745 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...quantifiable readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The...potentials; it’s an ion of interest for many disciplines! Most of the sensors designed for calcium generate...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson, 1982)....biological research, you might have an idea which ones could be helpful to you. Interested in learning ...Therapies in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001...

  3. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...difference (Figure 3). If you are interested in harnessing the power of digital PCR screening to validate.... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...through my work with the renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts... 3 topics and write them on a board to see which ones get the most hits. Commit to a meeting schedule ... get to present formally often enough) and give honest, critical feedback for improvement. Go to a science...

  5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene size ...to GFP-based sensors in terms of response and brightness. They also do not spectrally separate from green...(ex: tamoxifen). As the name suggests, CreLite combines the Cre/lox system with optogenetics. Cre activity...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large to ...Find the SEND plasmids    Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen...editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology paper...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... the same isotype. But together, all the B cell clones in the immune system make different isotypes of... starts with selecting just one of these B cell clones for further antibody production, polyclonal antibodies...antibody increases the likelihood that one of the clones will still recognize your protein. For the same...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...overcome by screening a higher number of potential clones. Golden Gate assembly has a few advantages over... allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct...ligated following a heat inactivation step. If your genes of interest or destination vector contain multiple...

  9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...malignant cell, then you’d need the ability to turn on genes at different times. It requires more complex synthetic...on an engineering project including hundreds of genes and regulation and resource control - everything...gives us enough hardware to control hundreds of genes. We’ve created enough regulatory elements, CRISPRs...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied... Selection marker while engineering viruses Acr genes can be used as positive selectable markers in engineering...viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...with those particles. Since the Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component...also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses need ...attention to the trainings!), even if you know guidelines for best practices and have been trained elsewhere...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...institute’s blog. The Wellcome Trust blog or the John Innes Student Voice are two examples. Many blogs like ...Londonist. BBC provides a peak into their day-to-day business with popular work experiences of 3-10 days. And...started writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography...

  14. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...Plasmids designed to express genes in a given host cell type are generally broken down into two broad...Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to Reduce Viral Titer in...Posttranscriptional Regulatory Element Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of ...

  15. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...management-for-scientists-managing-vs-leading. The National Postdoctoral Association (NPA) outlines 6 Core Competencies that all postdocs (but also...actionable tasks and I made a point of sticking to deadlines and performing the work I agreed upon (just as...accommodate many different types of people in order to harness their strengths and work effectively. Leadership...

  16. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...panelists will negatively affect timing and the liveliness of the discussion (see below on timing). Get ...what happens to the attention of the audience – phones come out, postures shift, they are checking out...more great tips on running panel discussions see Guidelines for Great Panels by Scott Kirsner and How to ...

  17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner, you...also quite efficient; although efficiency often declines as the number of fragments you’re hoping to assemble...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

  18. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available...measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791. https...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

  20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...Manuel Leonetti to tag all 22,000 protein coding genes in the human genome with fluorescent proteins. For...tool Crispor by Max Haeussler. With a few extra lines of code, we enabled it to return guides and primers...which is important for quality control. The only ones we found that worked with NGS data were: Crispresso...
Showing: 401 - 420 of 745 results