We narrowed to 507 results for: otos

1. Cultivating Community Science at BosLab

   Type

   Blog Post

   Published

   Nov. 2, 2017, 1:35 p.m.

   ...compounds in the environment, and openly publish protocols so that anyone can engineer yeast to do similar...

2. Science Career Options

   Type

   Blog Post

   Published

   Feb. 23, 2017, 1:30 p.m.

   ...Documentary Director Parasitology Botanist Nature Photographer/Artist Ecologist     Computer and writing...

3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...extremely high frequencies of HDR. The detailed protocol for nucleofection as well as the sequences of ...

4. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ...up (for example, notifying people of an updated protocol), send an email instead. The 3 A's - Agendas,...

5. Addgene Welcomes John Doench to the Advisory Board

   Type

   Blog Post

   Published

   Aug. 13, 2015, 2:30 p.m.

   ...bordering on research, rather than 'here’s the protocol, get a result and hope it’s close to the right...

6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...individual fluorescent protein (brightness, photostability, and so on; see the previous blog post for ...

7. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post,...

8. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...ligands, including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades...

9. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

10. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...position of having robust, null findings that are notoriously difficult to publish.  With Registered Reports...

11. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...

12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...

13. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...with the automation team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications...

14. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than ...

15. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...significantly stronger phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds...

16. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...A: At CLOVER, we have traditionally used qPCR (protocol link). We have found this method to be reliable...

17. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...

18. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...

19. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ... at first to look at so many plasmid maps and protocols! In reality, there are many shared components ...

20. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...to be due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson...