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Showing: 401 - 420 of 617 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted for their...explaining a gap in your job history or sharing relocation plans. They can be skills-based: if you're switching...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... so any results generated are reproducible and reliable. When you order a new cell line you will need ...conducted during projects to ensure the results are reliable. With this two-pronged approach, the many mistakes...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...literature to find all the applications of optogenetics relevant to your field. Ultimately, your question should... compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...a fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence...on collateral cleavage by the enzyme and instead relies on its activity on the target regions thus making...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...question? This should be something that anyone could relate to, that people would agree is important to understand...

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...integration for their life cycle to continue. They rely on a viral protein called reverse transcriptase ...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells and...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version...measurement of different RTKs. Although this sensor relies on a change in localization to the cell membrane...

  11. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

  14. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...etc. This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help... try to keep conversations focused on research relevant objectives and their tangible resources. For example...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

  16. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...and test multiple mice with the drug rather than rely on the results of a single mouse. Biological replicates...

  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...
Showing: 401 - 420 of 617 results