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...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

...practices for assemblies of more than 10 modules often rely on two-step hierarchical approaches using different...pre-determined manner. If the original insert religates into its original vector, it will subsequently...

...recombinantly append their proteins of interest with unrelated peptides that already had high quality antibodies...within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches” was not...

...volume. In many biological research situations, the relevant volume is that of the cell. For the physical behaviors...determine if an FP will dimerize in an experimentally relevant circumstance. For a more detailed discussion see...

...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...

...AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease in humans (and no vaccines...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...Designing donors for HDR The basis of the HDR pathway relies on repair from a template molecule, which is usually...

...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

... One key difference is the plasmid DNA must be released from the bacteria in order to serve as PCR template...reaction. You just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample...

...driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to a ~4.5 Mbp region...required the same receptors that its parental AAV9 relied on for transducing cells: galactose and the AAV...

...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

... important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted for their...explaining a gap in your job history or sharing relocation plans. They can be skills-based: if you're switching...

...forward. Communication is key as ideas must be relayed quickly both internally and externally to clients... tools to a consulting team. Your case team will rely on you to bridge the gap between the science and...

...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small...

... creating a robust data pipeline that would be reliable and available for our teams to use, one of our...container for its particular purpose and knew we could rely on the containers to produce the same output regardless...

...works with Addgene in some way. For users, we just released a major redesign of how search works on our site...sprints is themed and the stories in that sprint relate to the theme. For example, we could have a shipping...

... so any results generated are reproducible and reliable. When you order a new cell line you will need ...conducted during projects to ensure the results are reliable. With this two-pronged approach, the many mistakes...

...literature to find all the applications of optogenetics relevant to your field. Ultimately, your question should... compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation...

... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...question? This should be something that anyone could relate to, that people would agree is important to understand...