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We narrowed to 628 results for: rel

Showing: 401 - 420 of 628 results

  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...Designing donors for HDR The basis of the HDR pathway relies on repair from a template molecule, which is usually...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease in humans (and no vaccines...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... One key difference is the plasmid DNA must be released from the bacteria in order to serve as PCR template...reaction. You just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small...

  10. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

  11. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... important. On the other hand, our app/dev team rarely, if ever, sees cover letters submitted for their...explaining a gap in your job history or sharing relocation plans. They can be skills-based: if you're switching...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... creating a robust data pipeline that would be reliable and available for our teams to use, one of our...container for its particular purpose and knew we could rely on the containers to produce the same output regardless...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...works with Addgene in some way. For users, we just released a major redesign of how search works on our site...sprints is themed and the stories in that sprint relate to the theme. For example, we could have a shipping...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...forward. Communication is key as ideas must be relayed quickly both internally and externally to clients... tools to a consulting team. Your case team will rely on you to bridge the gap between the science and...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... so any results generated are reproducible and reliable. When you order a new cell line you will need ...conducted during projects to ensure the results are reliable. With this two-pronged approach, the many mistakes...

  16. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...literature to find all the applications of optogenetics relevant to your field. Ultimately, your question should... compared to controls without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation...

  17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...a fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence...on collateral cleavage by the enzyme and instead relies on its activity on the target regions thus making...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...question? This should be something that anyone could relate to, that people would agree is important to understand...
Showing: 401 - 420 of 628 results