...requires genes to be regulated dynamically.” To understand how that works in memory formation or other brain...cells but extends to freely moving animals, as established for conventional optogenetic tools." In total...applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic control of ...players in normal biological processes and disease states. While LITE plasmids have clear application to ...mammalian endogenous transcription and epigenetic states." Nature. 500, 472–476 (22 August 2013).   , ...

...enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment to open...complement our next-generation sequencing, setting new standards for plasmid quality control. Through our partnership... research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024; Kahn et ...conversations both online and in-person, you helped us understand how Addgene can better serve the global scientific... how you receive plasmids — as DNA or bacterial stabs — ‌and reinventing our search experience so you ...

...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...We borrowed an idea from the software folks and started Hack Days. During Hack Days, teams at Addgene will...Executive Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice...wonderful tradition for university departments to establish, with the goal of fostering better communication...we head into Thanksgiving weekend in the United States, I look forward to gathering around a large table...

..., we discuss some of the many things you can do start your own science communication student group and... and Amy say is the quickest way to get things started? Set up your own Science by the Pint series and... the SITN podcast as a means for listeners to understand the dynamics of information flow. Some of the... best way to get a Science Communication group started. It's as simple as getting people together at a...email [email protected]    For more info about starting your own SciComm organization and to learn about...

...As we get closer to the start of another academic year, graduate students and post-docs alike are wondering...ourselves credit for what we have accomplished. Starting with a recap of accomplishments, from learning...with lab members, determine how to work through obstacles, and take steps to resolve interpersonal conflicts...technique are beneficial for researchers at all stages. Rather than succumbing to academic inertia, it...'t Just More Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD...

... process will look slightly different from our standard process. We’ll walk you through a brief overview...be able to request Packaged on Request service starting in January 2025.  What will the ordering process...flexibility; while ensuring we can maintain high standards for both our depositors and requestors.  Requesting...specialist will reach out to you during the request stage, before you order.  Timeline Once your order is ...place a request and order if approved.  I want to stay updated!  We want to keep you updated! Subscribe...

...but many throughout the world are affected by devastating diseases that affect far fewer people and therefore...provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi - Spinal muscular...expression in specific cells. With a more concrete understanding of the molecular biology behind SMN1 and its...screens. He plans to work with a partner at IRCCS Besta in Milan to test small molecules targeting SMN1 ...Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated with the University of...

...many of the speakers.  As Dr. Polka said as she started things off, “We are all capable of contributing....  His list is dire:  The current system is unsustainable, hypercompetition is corroding values, we are... away from PI driven basic research, there is resistance to change, public support is weak, government...pretty much said it all.  She cited the oft quoted statistic that only 15% of current postdocs will be academic...drastic change in the way we train scientists to start addressing this problem.  Finally, one point made...

...come together over the past few years to propose standards and recommendations for improving antibody use... is all it will bind to - particularly when you start using complex samples such as whole cell lysate ...be aware of any possible alternative translation start sites or alternative splicing that could lead to...bound to the target protein, so it’s important to understand if protein:protein interactions could be a factor...Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use antibodies from Addgene Designing...Bradbury A, Plückthun A (2015) Reproducibility: Standardize antibodies used in research. Nature 518:27–9....

... longer than necessary before starting your experiment, or if you start an experiment before adequate ... or frt-STOP-frt (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the...Therefore, the first step in any AAV experiment is to understand which serotype best transduces your cells of ...the serotype, except now you will already have a starting “optimal” injection volume and titer to begin ...vectors in your experiments requires a full understanding of vector performance in your system. It is ...

...post was contributed by Jordan Ward who is an assistant professor at the University of California, Santa...elegans relied on selective markers such as drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur ...unedited animals struck me as a “needle in the haystack” type of problem and led to me to explore alternate...lethal mutation should remove much of the unedited “haystack”, facilitating recovery of edited animals. ...allowing novice researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more...Thank you to our guest blogger! Jordan Ward is an assistant professor at the University of California, Santa...

...this experiment? Can you follow and understand the protocol from start to finish?’" says Caitlin. SME’s...well.’” Stay tuned for the next feature in this series. We’ll be interviewing Susan Keown, a staff writer...!'" Working as a subject matter expert Caitlin started out as a subject matter expert (SME) at eScience...

...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...diseases. The “cause of disease” often requires understanding the disease genetics and the “treatment” usually...time and money gets put into them in those later stages. Target identification Not all diseases are fully...that target and validate the hit compound. The standard is to test the drug on models which are genetically...are selected.      CRISPR aids in multiple stages of the drug discovery pipeline.   CRISPR ...

...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...successful manager. Great bosses tell people where they stand clearly and routinely. They are clear with each ...questions after a task or period of work. Using this standard format not only enables you to discuss a person's...looking for a new position. One of the most common mistakes I see is managers who hint at or use indirect ...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

...community. In the long term, the research project establishes your professional brand. It offers colleagues... as a stepping stone into the next professional stage of your career by connecting you with people of ...plan? Have you read the relevant literature to understand where the field is today? Have you talked to ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...Choosing a Research Project Advice for Scientists Starting a Lab    ...

...expression, is it necessary to add a G nucleotide to the start of my target sequence? A2: The human U6 promoter...promoter prefers a 'G' at the transcription start site to have high expression, so adding this G could help ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...that mutation be selected/screened? A4: Before starting your experiment, consider co-transfecting with... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...

...antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves... editing, transformation may be identified by resistance to an antibiotic. Successful editing of transformants...requiring the destruction of plants or tissue for GUS staining or are not suited to studying large samples. RUBY...may contribute to the emergence of antibiotic resistant disease. (RUBY-red: You can’t beet it!) About...

...use, and likes to start at the beginning, shepherding a new product from the idea stage through all of its... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were... plasmids contain features such as antibiotic resistance markers and replication origins, but there is...efforts, such an algorithm has limitations. For instance, it could miss a common feature if the sequence...

...the plasmid here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material...Golden Stopwatch Award recognizes those TTOs with outstanding material transfer agreement (MTA) turnaround ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...possible new awardees, and we’re happy to share stats and strategies with tech transfer offices. Drop ...Sloan-Kettering Cancer Center  Monash University  Montana State University  National Institute of Diabetes & Digestive...

...and all the shortcomings and mistakes pointed out. Therefore, I try to stay away from the loaded and judgemental...ensuring that published work meets the accepted standards of reporting in the discipline and reduces the...underscore the sentences that I found hard to understand or individual words that are not appropriate ...whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...