We narrowed to 460 results for: try

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ... Stefan Hell, who won the 2014 Nobel Prize in Chemistry for his contributions to super-resolution light...

2. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...

3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...and suggestions, and even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive...

4. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff. ...

5. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives the loxP site directionality...

6. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...

7. Hot Plasmids - October 2020

   Type

   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...

8. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

9. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...   Confirming the gene/insert by aligning to NCBI entry or using BLAST.   Confirming tags and fusion proteins...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These...

12. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

13. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston...

14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...Regulation by Long Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146...

15. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....

17. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...

18. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...

19. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...