We narrowed to 783 results for: INA

...Nobody EVER complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts... at [email protected]." You can find his original blog post below! --Ed.  Eight years ago, I decided...proper consideration. 3. Present an easy to follow seminar See item 2. Attendees will include grad students.... This isn't the time to go into detail. Finally, state how successfully completing the research...applying for It's not about you and how you will finally be granted the faculty position you have craved...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves the supplied...extracted from a sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially...amplification is done at one constant temperature, eliminating the need for thermocyclers. This is followed ...contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence...

...short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher...strand invasion, a key step in the homologous recombination pathway. The Waldman lab at the Georgetown University...for the generation of knockins via homologous recombination with a homologous repair template. It is this...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele... mechanisms and consequences of cohesin gene inactivation in human cancer.     References 1. Russell, ...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

...-open. In the two closed states, integrins are inactive, with low affinity for ligands. When integrins...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...into two groups. Seven are reconstructions of the original hybridomas as new reproducible rat or mouse rAbs.... Based on the established applications of the original hybridoma monoclonals, we anticipate that our ...other RGD-binding integrins, individually and in combination, showing that among the three αV integrins expressed...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...

...1977, Sanger sequencing, also known as chain-terminating or dideoxy sequencing, was a major breakthrough... of all possible lengths because extension is terminated upon the incorporation of a ddNTP. Researchers...gels are classic techniques that allowed for many seminal scientific discoveries (5, 8). While newer techniques...techniques. For example, restriction mapping led to recombinant DNA technologies that are now commonplace in ... Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors." Proceedings of the National Academy...al. "Large-scale and automated DNA sequence determination." Science 254.5028 (1991): 59-67. PubMed PMID...

...transferred to a membrane, stained with antibodies, and finally visualized.   This process allows you to detect...typically do not transfer as well using this method.  Finally, commercially available dry blotting systems efficiently...materials and methods sections of those publications.  Finally, always verify that the chosen antibody works in... these cases, the western should be repeated.  Finally, while westerns are a useful way to gauge protein...phosphorylation, acetylation, methylation and ubiquitination. For example, to test if a protein is phosphorylated...and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–...

...the billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel...enterprise towards greater reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers...so-called “negative” results that conflict with our original hypotheses. Looking for novelty in our stories...similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000, PeerJ... presents only the results that align with the original hypothesis. Other practices that fall into this...researcher would get in-principle acceptance of the final manuscript regardless of whether the ultimate results...

...methods in previous blog posts, like developing recombinant antibodies, antibody conjugation, chimeric antibodies...of an antibody but want to know if different combinations or even different chimeras might function better...full-length antibody sequence, but with random combinations of the different sections. Structure-guided ...IPI) provides information on their large-scale recombinant antibody production platform. IPI is a leading...scientists. Addgene partners with IPI to provide recombinant antibodies that target families of cell surface...and Search Engines Antibodies 101: Producing Recombinant Antibodies An Integrin Antibody Toolkit from ...

...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...CRISPR/Cas9 to create mutations that essentially eliminate expression of the target. Removing (or reducing...interest in your sample, should lead to reduced or eliminated antibody signal. If you see a similar antibody...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...results every week always came with surprises. Originally we thought during the first three weeks that ...capstone project report and presentation, during the final week at Addgene. This report shared my work by breaking...each day, my background, observations, etc. On my final day (today!), I presented my research to Addgenies...