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We narrowed to 772 results for: INA

Showing: 211 - 220 of 772 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...transferred to a membrane, stained with antibodies, and finally visualized.   This process allows you to detect...typically do not transfer as well using this method.  Finally, commercially available dry blotting systems efficiently...materials and methods sections of those publications.  Finally, always verify that the chosen antibody works in... these cases, the western should be repeated.  Finally, while westerns are a useful way to gauge protein...phosphorylation, acetylation, methylation and ubiquitination. For example, to test if a protein is phosphorylated...and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–...

  2. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...the billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel...enterprise towards greater reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers...so-called “negative” results that conflict with our original hypotheses. Looking for novelty in our stories...similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000, PeerJ... presents only the results that align with the original hypothesis. Other practices that fall into this...researcher would get in-principle acceptance of the final manuscript regardless of whether the ultimate results...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...Gautier et al., 2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D. K... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes...

  4. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...CRISPR/Cas9 to create mutations that essentially eliminate expression of the target. Removing (or reducing...interest in your sample, should lead to reduced or eliminated antibody signal. If you see a similar antibody...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

  5. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’...program, teaching me the basics of physics and sustainable design while allowing me to do my internship ...results every week always came with surprises. Originally we thought during the first three weeks that ...capstone project report and presentation, during the final week at Addgene. This report shared my work by breaking...each day, my background, observations, etc. On my final day (today!), I presented my research to Addgenies...

  6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited... more than 45 publications, beginning with the seminal research articles that demonstrated the feasibility... Each screen curated in ORCS is linked to the original publication via its PubMed identifier, which can...metadata.   Screen results are reported as the original quantitative gene-level scores provided by authors...rather than sgRNA-level sequence read scores. The original supplemental data tables available with the publication...

  7. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ... PMID: 30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein...Weinstein Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for...organism. The SPARC system uses competitive recombination sites to selectively remove a stop cassette ...effector is expressed. By truncating one of the recombination sites in the construct to different lengths,...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... the protein with enterokinase, which recognizes the 5 amino acids on the C terminal of the tag. Of the...background can be a huge challenge. Epitope tags eliminate the need for target-specific antibodies and have...genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you are fortunate...protein that can also be used as a tag! GFP was originally derived from jellyfish (Aequorea victoria) and...

  9. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies are plasmid-based and ...The resulting chimeric antibody would have the original variable regions from the parental rat antibody...antibody retains the binding specificity to the original target and can be used with secondary antibodies...is routinely used to increase the utility of recombinant antibody tools, the resulting chimera may have...

  10. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...single-cell sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring. ...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ... Christina Mork...
Showing: 211 - 220 of 772 results