We narrowed to 855 results for: NTS

...of DNA Elements Project (ENCODE), an NIH-funded effort to define all of the functional elements in the...similar. There may be other variants of this that are better for some experiments than those we’ve put up....Eric Mendenhall of the University of Alabama in Huntsville explains it, a major goal in his laboratory is...groups got together to adapt CRISPR for tagging experiments and it’s already looking like it’s making a big... system is really quite simple but it offers improvements in efficiency and throughput. In adapting the...developed their own system or who use and adapt our reagents to think about adding them to Addgene’s special...

... just that as part of their CRISPR work in rice plants. Yang was intrigued by the tRNA processing machinery... in eukaryotes (E in bacteria), and only a few elements of tRNAs are necessary for recognition and cleavage...addition, tRNAs contain internal transcriptional elements that enhance pol III transcription, potentially...also examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs... five gRNAs were tested, they found that 50% of plants had biallelic mutations at all five loci. This ...Although Xie et. al characterized the system in plants, PTGs will likely work well in many different biological... regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria and viruses...

...Mentoring Group all participants act as both mentors and mentees. Because the participants are at a similar...make mentoring less mysterious and train the participants in skills useful for future mentoring relationships...at the same time Broadens the network of the participants Can be a lot of fun! Form your own peer mentoring...mentoring group In my travels speaking to grad students and postdocs I have started to talk to trainees....g. all women, all men, all postdocs, all grad students) or mixed for one or both of these criteria. If...you can, include people from different labs, departments or organizations as it brings a diversity of ...perhaps a 3 hour breakfast once a month. All participants must commit to making the meetings a scheduling...

...We are excited to announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative...researchers, in addition to postdocs and graduate students as we have done in the past. Below, read about...Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect...post-transfection, excited at 505 nm (right). Scalebar represents 100 µm. Image from Abhi Aggarwal.   Brandán...providing insight into new methods for treating patients in recovery from opioid use disorder,” says Hubbard...line engineered to be electrically excitable. The mutants are co-expressed with a photoconvertible fluorescent...photoconverted, sorted, and sequenced to identify the mutants for further enrichment. Tian notes that this platform...

... from their native regulatory elements. Controls for RaPID experiments RaPID negative controls include...interest. See figure 1 below for details on the key components of RaPID. Ramanathan et al. also tweaked BirA... the role played by RNA-protein interactions in events that occur on a short timescale, such as during... BirA*-based affinity purification mass spec experiments. These background proteins were then subtracted...correlated with poor survival in breast cancer patients. By digging into the literature and analyzing ...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding... with increased serum iron levels seen in HHCS patients. These are just three examples of how RaPID can...

...Nature gives us even more to talk about, as it represents another step towards CRISPR germline editing ... the field will move forward from this work. Experiments & major findings Let’s start with the paper itself...forward with gRNA 1 to conduct microinjection experiments in S phase zygotes at 18 hr post fertilization...are needed for proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate would ...to correct the hype when we see it. This paper represents a huge advancement in our understanding of how...perform in embryos but also shows that many more experiments are needed before we can consider clinical applications...applications for germline editing. Although this paper represents a large step forward in the CRISPR field, it’...

... about postdocs. Among other involvements, he helped to establish grants through the Sloan foundation ...biomedical workforce. Quotes taken directly from participants (summarized in 2) touched upon many problems...hired to produce results, not scientists”  These comments are well correlated with published data. For example...better solution would be to reduce the number of entrants into PhD programs to more closely reflect the ... market for PhDs Later this week, postdocs and students will again gather in Boston - this time with the...arguing against reducing the number of graduate students accepted in STEM fields (6). Melanie Sinche ...PMC4484856. 5. Bourne, Henry R. "A fair deal for PhD students and postdocs." Elife 2 (2013): e01139. Pubmed ...

...(electronic table of contents) alerts from journals you read often. The contents of each issue will be...explain some of the rationale behind the line of experiments, what data they collected and what it means. ... model or overall conclusion. In others, the experiments are geared towards eliminating possibilities ...deeper about the data they are showing. Are those experiments the best way of figuring out what’s going on?...controls? Refer back to the methods section for finer points of the protocols. Are there any red flags about... days of file cabinets filled to the brim with reprints and papers with scribbled notations in the margins...searching, reading and organization tips in the comments below. Additionally, read more of the Beginner...

...Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic...of off-target expression and ways to optimize experiments to minimize it. Overview of DIO/FLEx vector design...problem of DIO/FLEx vectors, the cause of these events, until recently, was not known. A recent paper ... both the serotype and promoter with in vivo experiments to determine the optimal/minimal injection titer...interest. You should now be able to make smaller adjustments to a set of injections into your animal model...may be tempting to skip some steps and get to experiments. However, by optimizing all conditions first,...results.  Conclusion Using AAV vectors in your experiments requires a full understanding of vector performance...

...). If you are wondering which virus fits you experiments best have a look at this viral vector overview...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...screening of thousands of genes in genome-wide experiments. Gamma-retroviral vs. lentiviral vector systems.... More detailed descriptions of the lentiviral elements and the 2nd and 3rd Generation Lentiviral Systems...and tips for using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral...can be used to induce site-specific recombination events and is widely used in the field of mouse transgenics...Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org for the...