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Addgene

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We narrowed to 599 results for: REV;

Showing: 211 - 220 of 599 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...settings where antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies...Infection with the lytic phage kills bacteria not previously infected by the lysogenic phage, rendering the...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen...heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve for...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and... et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment...excitingly, assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with...

  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos, mesothelioma...This drug (known as an angiogenesis inhibitor) prevents the growth of new blood vessels required to sustain...treatment get, there still remains the problem of prevention. Asbestos exists pretty much everywhere, still...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors...reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in vitro applications...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several unique properties...adopts an enzymatically “active” state rather than reverting to an inactive state like Cas9 or Cpf1. As a result... Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... in a separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical...years on: the good, the bad and the ugly."Nature Reviews Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating ...P. Bentley The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable...the cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins...

  9. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... details. You DO NOT need to present a detailed review of your field or the finer points of your techniques...avoid the temptation to provide too many details. Reverse engineer your talk. Identify the key points you...This means that once you get an interview, your previous success will not necessarily translate into getting...

  10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...the problematically low fluorescent intensity of previous versions of Brainbow. Developed from Brainbow ...visual system, which has been difficult to study previously (Hadjieconomou et al., 2011). Cell tracing in...distances across multiple fields of view, whereas previous Brainbow technologies were useful for discriminating...
Showing: 211 - 220 of 599 results