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We narrowed to 792 results for: comp

Showing: 421 - 440 of 792 results

  1. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout et al.,...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...through biomedical research. The Camelid family is composed of camels, llamas, and alpacas (and their undomesticated...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...blog over the years and have described how they compare to other antibodies and affinity reagents. But ...

  3. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...change in pathology. If the disease pathology is complex, a model may be most effective. Once drug candidates...and generate physiologically relevant models. In complex diseases, it can aid in generation of multiple ...specificity of that target and validate the hit compound. The standard is to test the drug on models which...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...of this effort, members of the Rawlins Lab have compiled a new toolkit for performing genetics studies ...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS...in high throughput screening for agrochemical compounds that have the potential to regulate the parasitization...

  5. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

  6. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ... expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits and digital...with faculty members to make sure the courses encompass what they want to teach.” Besides working with...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...transfer in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required...efficiencies of stable gene transfer using SB100x were comparable to stable transduction efficiencies of integrating...

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...foremost, from the custom software used to design complex genetic circuitry to the downstream output. Tuesday...digital and analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also...

  9. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...make sure your plasmid and E. coli strain are compatible! Check out this list of common E. coli genotypes...

  11. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major step...long-read cDNA sequencing and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high... restricted spatial tagging), to confirm the composition of proteins found at the telomere, and hope it...

  12. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...

  14. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  15. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ... of the world was locked down, a South African company wanted to test if a type of antibodies from an ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...need help with your scientific research. It’s completely free, of course. You can find more information...

  16. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...

  17. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    .... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  19. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...

  20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...snaking through our cells’ nuclei--our chromatin is comprised of thousands of loops. These loops not only provide...
Showing: 421 - 440 of 792 results