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  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

  2. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

  3. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...degron will behave with a new protein, but you can make an informed choice! References and Resources References...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

  15. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

  22. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating...

  23. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...

  25. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

  26. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  27. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

  28. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

  29. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...
Showing: 631 - 660 of 702 results