We narrowed to 792 results for: Arch

...useful and most importantly, effective. In the research world, there is a myriad of tools this could be...intended target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field ...about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256...

...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...

...site-specific knock-in tool that can be harnessed by researchers for many applications. Your favorite Cas enzyme...generally low; to increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate...repair pathway choice. Shrivastav M, et al Cell Research (2008). PubMed. Mechanisms of DNA double-strand...

...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

...variety of organisms.  While much of current CRISPR research focuses on its potential applications for human...engineering tools that are available to academic researchers through Addgene. CRISPR components for plant ...promoter and gRNA scaffold sequence and allow researchers to insert targeting oligos with minimal cloning...

...decades since this transfer was first proposed, researchers have shown that these transplanted mitochondria...and altering the composition and dynamics. The researchers demonstrated this perturbation interrupted the...license.  In addition to chromoproteins, the researchers developed a version of the kit that uses melanin...

...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...

...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...

...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...

...antibodies means improving research. More specific antibodies support common research tools like western blots... Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing Recombinant Antibodies...

...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...

...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...

... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...

...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...gene of interest with these tools. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...

...efficiency. Together, these factors prevent stem cell researchers from generating clonal knock-in lines, especially... allele. For N-terminal tagging, specifically searching for error-free knock-in at both alleles may be...for Regenerative Medicine (CRM), Edinburgh. His research interests include optimizing CRISPR/Cas9 genome-editing...

...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...

...during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute...in plasmid purification kits Kit Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced...recycled materials Sustainable forestry certification Starch-based compostable bags for kit components Recyclable...

...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

...interesting view into how rapid innovation affects the research community. I’ve likened it to the celebration ...currently at in the CRISPR software space. The CRISPR research community hasn't had a chance to develop a consensus...technology has been surrounded by a whirlwind of research activity and development since its inaugural year...

...say thank you to all the many scientists and researchers who deposit with us!  This year alone, we had...Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized Depositor Shout-...