We narrowed to 575 results for: BASE;
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TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics...
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Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....Found in surgical suites, air fields, and military bases, checklists are the ultimate tool for doing things... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....expression. AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....consistent with the cell type that was predicted based on the Atlas. They also looked at 17 of these TFs... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....activation of transcription in bacteria by co-opting σ54-based transcription. Transcription from σ54 promoters ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated to capsid protein ... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m.... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....liability in respect to actions taken or not taken based on any or all the contents of this post. An attorney...September 2017, USCIS received 116,224 employment based green card applications and approved 91,023 during... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....calcium indicator, OCaMP (Aggarwal et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). ...scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists have developed calcium indicators... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....specificity. This can be especially important for base editing techniques, where Cas9 must be directed ... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m.... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform differently... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....is one of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101...