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We narrowed to 847 results for: CAL;

Showing: 421 - 440 of 847 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... molecular biology-grade agarose containing physiological concentrations of salts and glucose. If we circled...fluorescent protein), fluorescence microscopy can theoretically be used to select specific wells from the 48... terms of growth rates, morphology or other physiological properties. An advantage of this technique is...properties, either initially, or using secondary biological screens. For example, for cancer researchers,...Neurobiology and Anatomy at the University of Maryland Medical School in Baltimore. She is particularly interested...

  2. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...stability, and buffer compatibility have placed practical limits on single- or two-step assemblies. We have... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers... plate Fidelity of assembly (percent correct) Calculated colony totals Per 2 μl assembly reaction Per...outgrowth volumes described in Figure 3, with calculated yields from entire outgrowth built from 2 µl ...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Preger et al., 2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289...research, which required protein compartments to co-localize multiple enzymes in a reaction pathway, the MPyV...expression selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-mediated transduction of gRNA...Gonçalves and his colleagues at Leiden University Medical Center are now available at Addgene along with ...and very efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. ...and used AdV vectors to edit genes in several biological systems. It has been used for instance in inherited...Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 ....

  5. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than enough DNA to...be viewed more as a guideline, since they are typically based on the copy number of the empty plasmid ...improved plasmid DNA yield. Culture conditions play a critical role in plasmid DNA yield. Different plasmids ...are generally, but not perfectly, correlated. Typical growth times for high copy plasmids in standard...miniprepping at various time points. Measuring the optical density of the culture can indicate the number ...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...? The available filtering options now change dynamically based on the type of material and specific search..., followed by a convenient table of plasmids specifically designed for this purpose: Still stumped? Ask...results at all, let us know! Contact our scientific technical support at [email protected] and let us help.  ...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... science teachers at our school put out the call to local parents who would feel comfortable speaking ...parents who can answer some of the surprisingly technical questions elementary students can ask. It doesn... dangers of people using the technology in an unethical manner, but I also tried not to be an alarmist...fields in biology, are moving so quickly that it’s basically impossible for textbooks to keep up. Even the ...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... of these techniques have in common? They all typically require secondary antibodies, frequently of the...are derived from an unusual type of IgG antibody called a heavy chain antibody (HCab), which are unique...different species, nanobodies are useful for colocalization labeling of up to 3 targets (Pleiner et al ...desired fluorophores. In figure 4B below, triple colocalization is possible even when staining cells with three...multi-color staining workflow yields similar localization patterns as cells stained for one target (compare...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Ample distribution channels both digitally and physically. Easy to incorporate visuals if needed. Traditional...means of scicomm. Digital or print distribution.  Typically limited to a single page of information. Primarily...levels and interaction. Can bring in things to be physically interacted with. Public speaking is a common ...internet access. High probability of real-time technical difficulties Exhibits Medium to high Audience...exhibit). No remote access; limited occupancy. Typically needs to be staffed. Mixed Media Medium to...

  10. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...especially in the sciences and by extension to scientifically oriented nonprofits. Like Deb, I worked for...available through not-for-profits, universities, local and national government offices, schools, healthcare... arts? Do you find satisfaction in helping your local food shelf raise money? Do you have experience with... as a grant writer and/or grant reviewer with a local nonprofit that aligns with your passion to get grant...Join the Grant Professionals Association (GPA)  local GPA Chapters, and the National Grants Management...

  11. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...administered systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically...developed, funded, and shared has no doubt changed drastically over the past few decades. But no matter how ...and transgenic mouse lines for genetic access to cortical cell types. Cell. https://doi.org/10.1016/j.cell...

  12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ... an exciting class of compact genome editors, typically only around 400 amino acids in size. To give a...target DNA, TnpBs also need a short sequence motif called a TAM (transposon-associated motif), which is positioned...familiar targeting mechanism. Small RNA elements called right-end RNA (reRNA) or omega RNA (ωRNA) guide... a single experiment (Karmakar et al., 2024). Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The...The process for cloning the 20-nt guide RNA is identical for both vectors. Here, we provide a step-by-step...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...allows for directional assembly of DNA sequences, called modules or parts. Assembly is dictated by four-...for fresh bacteria. Performing your Assemblies Calculate your volumes As you progress through the MoClo...increase your ligation efficiency, we recommend you calculate the right volume of each plasmid according to ...the equation in Figure 3.    Figure 3: Calculation for the plasmid volume for MoClo experiments....high-throughput, we recommend transforming into chemically competent cells in a 96-well PCR plate. Always...

  14. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...production of viral vectors typically involves shuttle vectors, also sometimes called transfer vectors. The ...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course...host range plasmids shuttle vectors? While it’s technically accurate to say that shuttle vectors have a “... “broad host range,” these terms are typically used in different (though overlapping) ways. Broad host...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...product purity of 66%. Pro tip! Abasic sites are also called apurinic or apyrimidinic sites and abbreviated ...are the same protein!) (Chen et al., 2024). They called their initial editor AXBE, for A → X base editor...in a G → Y (Y = T or C) base editor, which they called glycosylase-based guanine base editor (gGBE) (Tong... Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene. gTBE can introduce...site A DNA site with the nucleobase removed. Also called an apurinic / apyrimidinic (AP) site. UNG / UDG...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an ...specified by the crRNA sequence, it adopts an enzymatically “active” state rather than reverting to an inactive...propensity of Cas13a to bind and cleave RNA non-specifically. The ability of dCas13a to bind target RNA without...modulating splicing, and regulating expression of specifically targeted RNAs. Finally (and perhaps most interesting...observed shRNA off-targets. They subsequently used catalytically inactive dLwaCas13a as a programmable RNA-binding...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were reported...current knowledge. The Abudayyeh-Gootenberg lab (also called the AbuGoot lab), a joint lab run by Omar Abudayyeh...originally suspected that this new Cas7-11 might be phylogenetically classified as the more complex Class I systems...switched up the serine integrase for one which can enzymatically take a genetic sequence and integrate it into...

  18. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically Targeted...Why pseudotype? Lentiviruses and retroviruses typically target immune cells with their wild-type envelopes...out this review article published in Reviews in Medical Virology (Li et a., 2018).   Figure 2:...6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006) Retrograde neuronal tracing with a deletion-mutant...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...studying biochemistry at the University of Southern California. He is also an intern at the Yang Lab of the ...transposon-encoded CRISPR/Cas systems in bacteria, specifically on optimizing strains with low efficiency.   ...of Sciences. He joined Shanghai Institutes for Biological Sciences in 2000 and became a professor in Synthetic...reprogramming of cells to understand the genetic and biochemical mechanisms behind their distinct phenotypes and...generation of engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered the Sheng...

  20. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...regions and allocate specific functions to them. Electrical recordings and lesioning studies have been essential...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...an enhanced propensity for movement and activity called locomotor sensitization. This enhanced movement...responsible for the antidepressant effect. To specifically activate the Drd1 neurons, they injected AAV2... "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress."...
Showing: 421 - 440 of 847 results