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We narrowed to 845 results for: LEA

Showing: 421 - 440 of 845 results

  1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...the promoter region of gamma hemoglobin (HGB1/2) leads to continuous expression of fetal hemoglobin even...efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of ABE8.20-m to edit primary...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...Addgene blog Find more blog posts about base editing Learn about another evolved base editor: ABE8e Read about...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...Addgene blog Learn about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko...environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown individual genes...-CRISPRi. As a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 ...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...desired recombination event will remove NeoR only and leave exon 2 floxed, as seen in line 4 of Figure 4. Since...tools for creating knockout animals or cell lines, please consider sharing them by depositing with Addgene... with CRISPR through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes On...Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR  ...

  4. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...serotype, as some rAAV serotypes are abundantly released into the culture media, while others remain cell-associated...and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the...and dehydration (Howard & Harvey, 2017). You can learn more about common AAV applications by reading our...Wilson, J. M. (2010). Efficient serotype-dependent release of functional vector into the culture medium during...

  5. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...four times in a 95% ethanol and dry ice bath to release the virus particles from the cells. Cell lysates...batch-to-batch variability. Check out this post to learn more about different ways to titer AAV. Do you ...you have any AAV production tips or tricks? Please share them in the comments below! Special thanks ...first time CRISPR user Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out...

  6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ... blog Learn the basics of Golden Gate assembly Check out NEB's work on ligase efficiency Learn about ...assembly, ligation of mismatched pairs of overhangs can lead to incorrect assemblies, so care must be taken to...is a molecular biologist and a Development Group Leader overseeing a team bringing new products to market...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...remember what to say and provide your audience with a clear picture of your work. Practice, practice, practice...with people inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your...Anticipate questions: When practicing with your colleagues, ask them to brainstorm potential questions you... of a member of the Addgene depositor community Learn about careers at Addgene ...

  8. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...communication. But first, take a deep breath; at least you know you have an answer to that previous nosy... complicated process even more difficult. I’ve learned that some of the technologies that helped us navigate...application process and usually occurs in the few months leading up to applications opening. With virtual options...be a great opportunity to introduce yourself and learn more about your schools of interest. Benefits of...

  9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...forming noncovalent dimers. This property of EGFP can lead to significant artifacts. If you're using green ...reportedly monomeric FPs are not actually monomeric, at least in practical terms. The propensity of a particular...FP dimerization coupled with POI dimerization can lead to polymerization of the fusion protein. A dimeric... members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...responses that inactivate viral genomes or otherwise clear the virus from the cell population (Kafri et al....Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

  11. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. These studies...sequence via a targeting gRNA. But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused...research conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii. References..., et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096...

  12. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...you are purifying plasmid DNA from bacteria or cleaning up a PCR product, a common material you are trying...protein contamination. However, this ratio can be misleading. Some reports suggest that the A260/280 ratio...reagents used during nucleic acid purification can leave behind a variety of potential contaminants, some...Remember to discard flow-through solutions and use clean tubes? How about spin times, were these long enough...

  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...research interests include proteomics, machine learning, and visualization for big biology. GPS for the...are critical? One hypothesis: the enhancers! This leads us to a three-step approach for designing an experiment...database. Aneesh's research interests span machine learning, proteomics, and user interfaces for math.     ...Catch Up on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...finding how-to videos on YouTube long before he learned (somewhat successfully) how to text or use Facebook... communication that you enjoy and that plays at least slightly to your strengths. Otherwise, you’re going...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...balance out cons. Flexible. Can allow people to learn in multiple ways from the same experience.  Can ...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...and controls (see Tips below), and by validating leads with other methods. More common are false negatives...levels of bait and prey proteins - overexpression can lead to false interactions. Lowered expression levels...techniques! Minimizing false negatives: Interactions leading to detectable reporter signals depend on a number...problems with one or more of these parameters can lead to a high number of false negatives in Y2H screens...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...a cooler. Chances are one of your colleagues has a frisbee at least. Use it! 8. Organize If you’re doing...make an effort to build relationships with their colleagues. It can be easy to get caught up in the minutia...true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate- it will...

  17. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ..., and they stepped up to apply for a grant which lead to their new career path.  A part of the career ...story for most established grant professionals is learning to succeed in grants through trial and error, ...increased their demand for stronger budgets with clear budget narratives, logic models, established partnerships... Throughout these moves and program changes, I learned to write grants for health, housing, economic development...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...visualization tool, a quadrant will often be too clean for real-world data. Most plots will have a shape...    Of course, there are many more things to learn about when diving into flow cytometry data. There...

  19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated...fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood...Episomal vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector... peripheral blood mononuclear cells...

  20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... proximity of the fluorophore and subsequently leading to a positive signal. The same effect can be achieved...this approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (...assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound malachite...Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with...
Showing: 421 - 440 of 845 results