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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 709 results for: MAK

Showing: 421 - 440 of 709 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

  4. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

  5. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

  7. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  13. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

  14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...chromoprotein detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if ...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

  15. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...
Showing: 421 - 440 of 709 results