...studying its structure can help us understand the parameters of CRISPR experimental design. Let’s dive into...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...

...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

...increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks...like to measure yours? More Reading Fenner M. "Altmetrics and Other Novel Measures for Scientific Impact...

...precipitation method or using a silica spin column available from several vendors. The precipitation method has ...

...of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science...

...CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...

... Cas systems, Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler ...they mined a database of microbial genome and metagenome from the Department of Energy’s Joint Genome ...

...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding...

... in terms of contamination, but consider flow cytometers. These fluidic machines handle biological samples...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...

... scientific research. Get an overview of these methods in this blog post. Hope you’ve enjoyed going through...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving...

...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...while some might argue that the only way to do something is to do it huge, there are times when a more ...

...this month, I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing...

...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...

... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...new x-ray technique provides scientist with a new method to explore the inner structures of delicate plants...

... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family...

... for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592...Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592...

...out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work...

...foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and eat, and...vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are...

...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is...Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical...

...fingerprint can be detected. Bourdeau et al calls this method acoustic collapse.  Importantly, acoustic collapse...have already developed a high-throughput screening method for serial acoustic collapse to aid in the development...