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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 421 - 440 of 711 results

  1. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...serotypes have a primary cellular receptor and sometimes one or multiple coreceptors, which may be present...present on different cell types and sometimes vary between target species or even strains. Pro tip! Getting..., A. C. H., Gokal, D., Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Molecular Therapy - Nucleic...hydroxylase promoter in vivo. Molecular Therapy - Methods & Clinical Development, 3, 16062. https://doi.org...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...multiplexed over 65 targets!) IF is a versatile method with endless possibilities, and there are always...Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke...

  4. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Crystal Violet Pre and Post Visible Light 100 Methylene Blue Post Visible Light 100 Ethidium Bromide...from the gel via your favorite gel purification method, it is important to determine the concentration...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...and volumes to determine the optimal injection parameters needed to transduce your cells of interest. Using...Once you have determined the optimal injection parameters for the serotype that best transduces your cells...performance in your system. It is very rare for a methods section to include all of the variables that need...

  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...studying its structure can help us understand the parameters of CRISPR experimental design. Let’s dive into...is recognizing the PAM on DNA, the CTD is also sometimes called the PAM-interacting (PI) domain. The guide...al., 2013). As mentioned above, however, Cas9 sometimes tolerates mismatches in the next 8-10 bases after...

  9. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov tells us...modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically...

  10. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks...like to measure yours? More Reading Fenner M. "Altmetrics and Other Novel Measures for Scientific Impact...

  12. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...CRISPR-based method for cell lineage tracing Valcyrie is a CRISPR-based marker excision method for marking...

  15. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...With the meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding...

  16. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ... in terms of contamination, but consider flow cytometers. These fluidic machines handle biological samples...markets. Rethinking our approach to lab waste is something we all need to do. Remember, it was only 50 years...

  17. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... scientific research. Get an overview of these methods in this blog post. Hope you’ve enjoyed going through...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving...

  19. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...this month, I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...taking a look at all of our posts as each one has something great to offer, I just need to get back to emailing...

  20. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...an indication of the stiff competition or says something about my cooking.   This is a creative (and delicious...Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice a year...
Showing: 421 - 440 of 711 results