We narrowed to 517 results for: PROC

1. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...

2. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ... and as societal participants in the political process. My role at pgEd I have always been very interested...

3. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...While this option is attractive because the same procedure for a single 10 cm2 dish can be easily applied...

4. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...maintain your parental strains throughout this process. In the F1 and later generations, mice that are...

5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...

6. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...to your parameters.   Kinetics How fast is the process you would like to monitor? Neurons, for example...

7. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...

8. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...have been cross-adsorbed. The cross-adsorption process filters out off-target antibodies increasing specificity...

9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...activation across multiple loci was an arduous process. When using zinc finger proteins or TALE proteins...

10. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting...

11. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...Schematic of the goldilocks antibody selection process The exposure time of the primary antibody compared...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...lines and use these lines to study how immune processes and biological pathways are altered in PMM2-CDG...

13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...

14. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...

15. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...overhangs. Exploring the efficiency of these two processes will help us better understand where Cpf1 fits...

17. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...

18. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...

19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...container before the progeny begin eclosion, the process of hatching from their pupal case (~9 days after...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...allowing them to optimize the compound production process. How have you used FP timers? Are you planning ...