We narrowed to 464 results for: SHI
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TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...
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Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....22368488. PubMed Central PMCID: PMC3279232. 10. Song, Shiping, et al. “Aptamer-based biosensors.” TrAC Trends... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....groucho gene? Check out plasmids deposited by the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....animals are engineered by introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... the non-specific proteins are removed through washing, the target protein can be eluted. The elution ... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....Panda, S., Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, Y., Nayak, A. K., Baig, M. J., & Molla... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....Hok translation is coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....the many individual stories and get (sometimes refreshing) insights into how other scientists think and... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m.... years later. While there is a dose-response relationship (the higher the exposure, the greater the risk... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....is 20 years old but as you can imagine not old-fashioned at all. With the development of new fluorescent... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....temperature, allowing for a flow analysis to be shifted to a later day. Note that intracellular labeling... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m.... Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....extended-open. When a ligand binds, it typically shifts the integrin from its closed to its open state,... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae, and Serratia marcescens... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...