We narrowed to 460 results for: SHI
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TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m.... years later. While there is a dose-response relationship (the higher the exposure, the greater the risk...
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Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....groucho gene? Check out plasmids deposited by the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....Hok translation is coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... the non-specific proteins are removed through washing, the target protein can be eluted. The elution ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....animals are engineered by introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....the many individual stories and get (sometimes refreshing) insights into how other scientists think and... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....used to refer to something that has fallen out of fashion but is still useful and most importantly, effective... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....PubMed Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....temperature, allowing for a flow analysis to be shifted to a later day. Note that intracellular labeling... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m.... Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....extended-open. When a ligand binds, it typically shifts the integrin from its closed to its open state,... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....cellular aspects of CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....double-strand break repair: how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae, and Serratia marcescens...