We narrowed to 580 results for: SIM

1. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...reporter. Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive...

2. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

   Published

   June 27, 2018, 1:16 p.m.

   ...Wanisch  Listen to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group...

3. Plasmids 101: Origin of Replication

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   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...

4. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   Published

   Nov. 12, 2019, 2 p.m.

   ...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans...

5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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   Blog Post

   Published

   July 7, 2015, 3:36 p.m.

   ...The data are clear - the SEC-based system is both simple to use and highly efficient for C. elegans genome...

6. Another Pathway into Cells: iTOP

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   Blog Post

   Published

   June 23, 2015, 8:37 p.m.

   ...instead of supplying a DNA construct, they could simply induce cells to take up protein directly? It’s ...

7. Summer SciComm Series: A PhD in Science Communication

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   Blog Post

   Published

   Aug. 23, 2022, 1:15 p.m.

   ...adeolitis” – received the best ratings overall, simply because none of the participants had preconceived...

8. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

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   Aug. 2, 2022, 1:15 p.m.

   ...thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed smear with...

9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   Blog Post

   Published

   June 23, 2022, 1:15 p.m.

   ...diseases with real phenotypic effect.” “It’s a very simple technology,” Doudna further explained. “That’s ...

10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type

    Blog Post

    Published

    April 28, 2021, 1:15 p.m.

    ...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

11. Reagent Repositories Are Speeding up Science During the Pandemic

    Type

    Blog Post

    Published

    Aug. 4, 2020, 1:15 p.m.

    ...murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV...

12. K. phaffii: Rising to the Occasion

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    Blog Post

    Published

    April 4, 2023, 1 p.m.

    ...evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...

13. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out...

14. Cultivating Community Science at BosLab

    Type

    Blog Post

    Published

    Nov. 2, 2017, 1:35 p.m.

    ...protocols so that anyone can engineer yeast to do similar things. The focus is to make yeast engineering ...

15. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    Published

    Sept. 28, 2016, 2:35 p.m.

    ... from otherwise terminally differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and...

16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...or in cell nuclei. The two photosensitizers had similar rates of off-target damage to neighboring cells...

17. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated...

18. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

    Published

    July 17, 2018, 12:23 p.m.

    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...

19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...manipulate that process. We began our research with a simple question: how do Cas9 and sgRNA interact with and...

20. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ...skills you developed through your research? Don't simply write about the research you did. Emphasize the...