...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is the ...DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another gene found ...cloning applications. While these two strains ultimately perform the same function, there is some evidence...

...copy numbers vary within cell populations and over time resulting in variable gene expression that can impact...expression of enzymes in a pathway will ideally be optimized to promote generation of the desired product while...Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains...

... and selective degradation of protein targets in time scales that are not possible with traditional genetic... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang et al.,...

... Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations...Connect with people you have really talked to The best time is within a few days after you’ve met. Always include...their profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant...

... possibly because in vitro conditions did not optimally support growth of diverse species. These results...deep sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome...containing the particle exponentially decreases over time and the number of elapsed generations can be determined...

...plasmids, viral service, and more For the first time in the last couple of years, we don’t have to worry...way. Scientists know this and recognize it all the time. We love this feedback and are always adding to ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular...

...antibodies and find….hundreds of options. With limited time and budget you cannot test them all, so how do you...citations. An antibody that has been cited thousands of times may be a safer bet than one that has never been ...free to make the decision by price point, shipping time, or other factors. Just remember that these are ...

...At the same time, an adjuvant such as KLH or Freund’s adjuvant is injected, which stimulates the overall...that they are inexpensive. Their short production time and the bulk purification steps keep the cost low...

... scientists and has saved countless hours of lab time (in both donating and receiving labs)” — Jim Woodgett...alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally invaluable resource. I...Addgene that has been distributed more than 100 times.   “I really like this award because you don’t get...

...push the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering... need the ability to turn on genes at different times. It requires more complex synthetic networks. Addgene...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

...in culture for up to 20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw...effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale over the course...mL of sample. The purity of the sample can be estimated by looking at the ration of DNA (260nm) versus...

...structural proteins, such as the MLV protein Gag, multimerize and spontaneously assemble into particles at ...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...

...editing, but when is the best time to shut off Cas activity? Using inhibitor timing experiments, the Doudna ... Additional Resources on the Addgene Blog First time CRISPR user? Check out this post. Learn how to Choose...

... vectors you can find and use them as-is, saving time and effort but risking the potential of them not...vectors (McGee et al., 2024). It reduces the amount of time to design and create a new vector to four days for...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

... track. If you don’t want to commit time and money to a full-time graduate course, UWE Bristol offers ...engaging with other science bloggers. If you don’t have time to maintain your own blog, you can probably write...

...types of people both above and below you. During my time at the NPA, there were many changes to the  Board... As I spoke about my work with the NPA, I was ultimately invited to join the Advocacy and Outreach Committee...research field should I decide to remain here.  In a time when it is so important for postdocs and graduate...

...systems include that virus production can be quite time consuming and that insect cells require demanding...proteins from plants however can be expensive and time consuming as plant tissues themselves are biochemically...cells and growing conditions often need to be optimized. Yeast cells are also known for hyperglycosylating...

...conference After using your elevator pitch several times at the conference, you might notice some trends....out if you’d like to meet someone and set aside a time to meet. Some conferences may also have a dedicated...there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour) and use the conference hashtag...

...questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags For about...ideal.  Unexpected connections Right around the same time that Munro and Pelham were devising their tagging...Schüchner, et al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility...

...or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role...high-throughput genetic screen, it is expensive and time consuming to individually generate gRNAs and homology...the Addgene Blog Learn about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins...