We narrowed to 813 results for: TIM

... where image processing algorithms for analyzing time-lapse images, quantitatively evaluating antibody...download the software for use, or they can modify and optimize the code, find and fix security-critical bugs,...the world don't have to start from scratch every time to pursue their scientific curiosity but can literally...

... negative selection in plasmid cloning.  But oftentimes, a selection strategy is not enough on its own...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...

...as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-...temperature range than BioID, which only functions optimally at 37℃. The increased efficiency of TurboID and...when they surface. But detection of the virus costs time and money, and viruses at low concentrations often...

...appropriate ligand to activate the G proteins. It is estimated that there are approximately 950 different human...signaling cascades as a response to the extracellular stimuli. Ligand dissociation from the GPCR allows for binding...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

...tend to ask similar questions, thus devoting some time to writing a solid personal statement will pay dividends...with your personal statement, while you make the ultimate case: why you? 7. Strength in numbers Encourage...clinch every opportunity for themselves. This is ultimately the wrong approach. It is very likely you know...

...It’s time to choose your own protein purification adventure. You want to purify your favorite protein...After binding YFP to the column, you wash several times to remove non-specific proteins, and then elute ...light switch could control a signaling pathway. Ultimately, development of more OptoNBs would allow for ...

... the zinc finger nucleases. Unfortunately, it is time consuming and difficult for investigators without...genetically modified cells in less than a month's time. Addgene: What are the implications of this kit ...flexibility in terms of the target site. Also, any time you create double-strand breaks, there is a possibility...

...antibodies and find….hundreds of options. With limited time and budget you cannot test them all, so how do you...citations. An antibody that has been cited thousands of times may be a safer bet than one that has never been ...free to make the decision by price point, shipping time, or other factors. Just remember that these are ...

...At the same time, an adjuvant such as KLH or Freund’s adjuvant is injected, which stimulates the overall...that they are inexpensive. Their short production time and the bulk purification steps keep the cost low...

...but are simple to pick up and work well the first time. But biology is complicated — so many different ...contexts (Figure 1). Together the features with optimal biological performance are described as Click Biology...left-over starting components. High rate constant— time is money and things move fast in biology. Slow reactions...

... scientists and has saved countless hours of lab time (in both donating and receiving labs)” — Jim Woodgett...alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally invaluable resource. I...Addgene that has been distributed more than 100 times.   “I really like this award because you don’t get...

... and selective degradation of protein targets in time scales that are not possible with traditional genetic... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang et al.,...

...cloning experiments a little easier. One of the most time-consuming aspects of traditional cloning is the ...DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another gene found ...cloning applications. While these two strains ultimately perform the same function, there is some evidence...

... possibly because in vitro conditions did not optimally support growth of diverse species. These results...deep sequencing and qPCR methods are too costly and time intensive to process a large number of microbiome...containing the particle exponentially decreases over time and the number of elapsed generations can be determined...

...plasmids, viral service, and more For the first time in the last couple of years, we don’t have to worry...way. Scientists know this and recognize it all the time. We love this feedback and are always adding to ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular...

...copy numbers vary within cell populations and over time resulting in variable gene expression that can impact...expression of enzymes in a pathway will ideally be optimized to promote generation of the desired product while...Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains...

...scientists frequently use growth factors or serum to stimulate a pathway of interest, but there can be a lot ...occurs when serum or growth factors are used as a stimulus. This system also allows precise modulating of...delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative feedback...

... Don’t Know This Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations...Connect with people you have really talked to The best time is within a few days after you’ve met. Always include...their profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant...

...push the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering... need the ability to turn on genes at different times. It requires more complex synthetic networks. Addgene...variation. The first one worked in each case. Sometimes in CRISPR people talk about off-target interactions...

...editing, but when is the best time to shut off Cas activity? Using inhibitor timing experiments, the Doudna ... Additional Resources on the Addgene Blog First time CRISPR user? Check out this post. Learn how to Choose...