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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 804 results for: arc

Showing: 421 - 440 of 804 results

  1. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... long tenure at Addgene. I would work with our research team and software engineers to update our Lab ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or...

  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...this process is not fully understood, a team of researchers lead by Takayuki Kogure (Kogure et al., 2011)...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little...are kind of hard to see. To solve this issue, researchers conjugate various labels to antibodies that produce...conjugation, which is commonly used for labeling research antibodies. Lysine residues are found throughout...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  8. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications...

  9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ..., Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, .../10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check...

  10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in...

  12. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...d like to modify. To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct...this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

  17. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... and what is their background relative to your research? Tailor your language to your audience so that...presenting at the American Association for Cancer Research Annual Meeting, you might consider including more...group who presented on a topic related to your research? Find a way to get in contact with the presenter...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your...all will be familiar with the specifics of your research. Try to avoid jargon in this initial introduction...data they may ask the audience to not share their research on social media. Use the hashtag if you are live-tweeting...

  19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus...sybody libraries in the form of mRNA for academic research. The template plasmids for generating your own...days. While the lab used this method for COVID-19 research, this protocol holds promise for developing treatments...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...depositing them in Addgene’s repository. Other researchers, eager to try this new technology, were quick...the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’re always...collection, we’ve shared almost 300,000 plasmids with researchers all over the world. That’s an average of 27,272...
Showing: 421 - 440 of 804 results