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... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

...creatine. In order to reach the brain, creatine needs to be transported from the blood and cross the blood...effector functions. It’s an important process that we need to understand so that we could possibly develop ...

...experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies to create...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted...

...Voigt’s group has also been assembling the toolbox needed for anyone to begin considering genetic engineering...identify and target a malignant cell, then you’d need the ability to turn on genes at different times....

...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

... you can show off your work at a conference, you need to first make your poster. While a poster generally... to verbally communicate your work, so you don’t need every little detail on the board.  Poster layout...

...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

...ABE8 (Gaudelli et al., 2020). The generated ABE8s needed to produce 3 concurrent base edits to withstand...editing single genes is very useful, sometimes, you need to modify multiple genes to generate a cellular ...

...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

...system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

... pose the greatest threat, cancerous cells still need to successfully infiltrate the circulatory or lymph...include several other important pathways. We invite feedback and contributions from all members of the research...

...recognition, which gives a researcher unprecedented freedom to target any sequence of DNA. Second, NgAgo target... by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells...

...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...