We narrowed to 533 results for: erf

1. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   Published

   Sept. 27, 2017, 1:23 p.m.

   ...talk – to win the audience’s applause with your performance. When your institute opens its doors to the public...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...

3. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment...

4. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...measurements are more susceptible to artifacts and pH interference, and comparison between experiments can be more...

5. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...SplitSet™. With these tools, NEB successfully performed a 35-fragment Golden Gate reaction, using four-base...

6. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...Regardless of which ligand you choose, there is no “perfect” drug that only binds to one receptor, so it is...

7. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...antigen in a shorter period of time and would likely perform better for probing poorly expressed targets. Similarly...

8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder...

9. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ...the resources to recapitulate the experiments performed by our thousands of depositors. That means their...

10. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ... FoR 2015 Symposium Learn to Advocate with the Perfect Science Talk Find a Good Mentor ...

11. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...regions of the genome, we have deposited the best performing sensors and constructs encoding corresponding...

12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...findings from structural studies on the ribosome performed in his lab. RNA is a structural and functional...

13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... be open to a wider group of people. This is a perfect starter project for our computer scientists, many...

14. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...problem by stating the facts and explain how it is interfering with your work. Avoid talking about your emotions...

15. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...good panel discussion is a well choreographed performance with room for fortuitous improvisation. You want...

16. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ... quantification.  Even though no one option is perfect, with all this variance in your variant options...

17. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition by specific...

18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...found in the culture media, you should consider performing PEG precipitation. Instead of sonicating the ...

19. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...cells. Baculovirus expression systems are very powerful for high level recombinant protein expression....

20. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...of the skin. While this could theoretically be performed anywhere on the body, somewhere large, flat, and...