...mentor (if you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting...the host university does not hire or pay them (typically). A visiting scientist is distinct from equipment...the scientist to the new institute where their typical workdays will be carried out until the end of the...equipment! Preparing for your visit   Design and communicate a research plan Your time at the new research... and you are set up for success. If you are communicating with a professor or institute, ask if you will...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ...Resources Resources on the Addgene blog Fostering communication in remote collaborations Transferable Skills...

... and 15/17 morphologically aligned. Documentation of the network that initiates biological reprogramming...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...published protocol and its relevant modifications on the pooled library webpage. Disease models Good disease...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...environment? Does the group expect each other to be physically present at certain times during the day? We asked... next.” Connie Cepko looks for people who are dedicated and interested in science. “Postdoc hires are ...history in science. Potential graduate students typically go through a rotation, which reveals their working...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ... "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing direction of...

...systems are available at Addgene! Chemically inducible promoters Chemically regulated promoters are among ...sensitive expression systems are typically less leaky than chemically induced promoters; they show near-zero...transcription is turned ON. Types of inducible promoters Chemical agents, temperature, and light are all examples...prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein...decreasing promoter leakiness.   Other examples of chemically induced promoters include positive inducible ... promoters only work in prokaryotic cells and typically only in the same or closely related species from...

...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program focused...BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer to pesticide... Dr. Natalie Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...and teachers to apply engineering principles to biological systems. Today, what started in Cambridge with...Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid summer internships...

...cost-prohibitive for many labs, and are often impractical for small projects. Most researchers instead ...involves splitting up a sample into thousands of physically isolated partitions (such as droplets or wells...Since there are many thousands of partitions, statistical modelling can be used to determine the actual..., does not require standard curves, and can theoretically detect a single target molecule in a sample....a gene of interest is one of the most common applications of precision genome editing, the remainder of...single molecule resolution of digital PCR allows classification of individual target molecules based on their...single wells of a 96-well plate without prior quantification. Comparatively, mismatch assays require between...

...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...psychrophilic enzymes for milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...cultivating a nuanced understanding of the societal ramifications of laboratory science, and how it may best be...

...to the right indicates a higher signal, or upregulation, and a peak to the left indicates a lower signal...anything to the right of it, marking it as GFP+. Quantification for such graphs could simply be the percentage...case for you, switching to a contour plot should indicate where the two populations diverge. The smear ...place your gate based on the dot plot would be complicated.  Figure 2: (A) a smeary dot plot showing...Figure 4 compared to the dot plot in Figure 2. Quantification using MFI makes it easier to identify and study...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...addgene.org Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...

...and success. Customer and technical support Our customer and technical service brands continue to ...to keep up with the demand with excellence and dedication. We don’t have worry or wonder if providing...projects allows us to support more new projects. Diversification is happening. The Addgene team will have some... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...we might do. I am grateful every day for their dedication, flexibility, innovative thinking, and openness...Addgenies support a high collaboration, high communication, high efficiency, high quality, low meeting,...about the Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to...

... microscopy. This application of AHA labeling is known as fluorescence noncanonical amino acid tagging...PhD students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this... symposium seeking inspiration to resolve the technical hurtle of monitoring protein turnover of specific...technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine (AHA). In the technique...DNA strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ...interested in neurobiology.  She is passionate about communicating science to all audiences. Right now, she is ...

...red fluorescent proteins) allow simultaneous identification of more than one protein of interest. However... species (bottom panel in Fig. 1). Our results indicate that the tag protein may have an important influence...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...However, in some cases the fluorescent signal may be critical for the experiment. Since the fused protein could...fluorescence expressed in the neuropil conceals identification of opsin-expressing cells). For other things...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper...Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex...

...repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time ..., 98% of TdTomato expressing cells were GFP+, indicating a robust, but specific system. T-DDOGs also successfully... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...Could Cas9-DOG be developed soon to allow genome modification only in a small subset of cells? Other proteins... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...

... work around this is to "domesticate" your fragment: PCR-based amplification can be used to create silent...and exciting applications, this system is an incredibly powerful tool for cloning complicated constructs...plasmid (Option 1) or added using PCR-based amplification (Option 2). Advantages of Golden Gate cloning...restriction sites that may not be amenable to "domestication", you might want to consider using an alternative...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...high-efficiency step. References: Sequential amplification of cloned DNA as tandem multimers using class-IIS...

...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In... become very different due to a whole genome duplication and extensive rearrangements in the S. cerevisiae...overexpression vector. This plasmid contains origins of replication and selectable markers that allow students to...functional MET genes to grow.) Students then use replica plating to determine if expression of MET/Met genes...met deficiencies (manuscript in preparation), indicating that Met protein functions have been largely ... Students who participate in the course show significant gains in both content knowledge and research ... research into undergraduate courses and in communicating science to the public. You can find out more...

...generated in vitro using synthetic genes that are typically expressed from a plasmid or from an integrated...cells, for antibody production and subsequent purification.  Academic laboratories and companies have already...share, making recombinant antibodies the most practical choice. Hybridomas are much tougher to ship, often... often lack sequence data, and may genetically drift over time. Antibodies made from animals are limited...the ease with which scientists can now improve genetically-encoded research tools, it is shocking that the...Begley CG, Ellis LM (2012) Raise standards for preclinical cancer research. Nature 483:531–533 . https:/...Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans ...

...production and subsequent necessary modification of these chemical products. The flexible nature of MoClo... Gate cloning is MoClo. That’s because MoClo specifically refers to the organizational schema described...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...together. They can express one or more gene(s), and typically begin with a promoter and end with a terminator...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor molecule...genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You...

...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...researchers to identify and isolate very specific biological targets. However, as popular and useful antibodies...produced from a new set of animals), and wrong application (different experiments/conditions can change ...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and accessible database for...antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are a relatively...a research assistant in lab of Zhuhao Wu at the Icahn School of Medicine at Mount Sinai.  References ...

...very useful in the event that you will not be physically present at your poster at all times, so other... sections should be as concise as possible, communicating only the most important results to your audience...have the advantage of being able to verbally communicate your work, so you don’t need every little detail...readers can navigate your poster and follow the logical progression of your work, and you can accomplish...offers free poster templates, although most are vertical.  Large central box. Another commonly used and...presentation. Portrait. This style displays a poster vertically rather than horizontally. Instead of displaying.... Shapes. In this type of poster, written and graphical information are inserted into larger circles or...

...Lacks - the African American woman whose cervical cancer cells became a mainstain of biological study without...advice on how to dive into new forms of science communication. Click here to listen to the interview with ...fossil rocks and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully...implement the next generation science standards and communicate science. Learn more about Tom on his youtube ...

...example, if you’re a virologist presenting at the American Association for Cancer Research Annual Meeting...therapeutic strategies to target it, than you typically would at a microbiology meeting. Create an outline...lab mates, and PI can provide feedback on your technical presentation, but those outside of your field ...don’t fully understand the question, ask for clarification. Having them reframe or elaborate their thought... through the list at the session so you can strategically use your time. Don’t have access to a list of...titles to find the ones you’d like to revisit. Strategically traverse the sea of posters If you come prepared...system, and is interested in a career in science communication.       Resources on the Addgene blog Read other...