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We narrowed to 678 results for: ids

Showing: 421 - 440 of 678 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... the community to share their expertise with the kids through some fun activities. If your school doesn...levels, and STEM exposure. If you expect a lot of kids, you’re going to want a lot of activities (try for...out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated...

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...ribonucleoproteins? One alternative approach, which avoids many of these complications, is to directly deliver... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs Check out our CRISPR guide...

  4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein..., transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure and...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...single population (e.g. transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid...the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID: 20719741. Pubmed...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...unintentionally limit the utility of candidate capsids.  Are you interested in using AAV-PHP.B vectors...permissive and non-permissive mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene...LY6A, a novel cellular receptor for AAV-PHP. B capsids." bioRxiv (2019): 538421. If you’re interested ...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding nor its ability...New York. pp 719. 3.Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking...Nathan C., George H. Patterson, and Michael W. Davidson. "Advances in fluorescent protein technology."...

  9. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even pathogenic bacteria...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...protein interacts with other proteins or nucleic acids. Read on to learn more about this versatile technique...used to study proteins that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...microbial contamination. Purity When possible, all plasmids used for viral production are propagated in the...endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using endotoxin-free plasmid... system and PCR-based confirmation of transfer plasmids used in Addgene’s viral production. Barcoding ...

  13. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...downsized by deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from...the development of non-natural fluorescent amino acids and their incorporation into proteins are steps ...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...

  14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... these mismatches. To detect SARS-CoV-2 nucleic acids, the target sequence is amplified by PCR/RPA using...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...by sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https:/...template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...flexible linker peptide that is usually 15-20 amino acids long and made up of glycine and serine with dispersed...display (Bradbury et al., 2011). In vitro selection avoids animal immunization and also allows for generations...treatment of HIV infection. Current Opinion in HIV and AIDS 10:190–197 . https://doi.org/10.1097/coh.0000000000000145...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such...edits, researchers have designed self-cleaving plasmids that liberate the targeting region from the vector... Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...

  18. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...study (1), we implement RSMs in E. coli with two plasmids (Figure 3 below): Input Plasmid: The input plasmid...two-input RSMs and three three-input RSMs. The plasmids used to construct these RSMs are listed in the... Visit Our SynBio Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  19. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...technique called Genome editing via Oviductal Nucleic Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/...
Showing: 421 - 440 of 678 results