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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 662 results for: mpo

Showing: 421 - 440 of 662 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...

  2. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...commercially available (see below). Conclusion It is important to note that thermostable, high fidelity, nick-selective... system for direct identification of allelic composition of mutated DNA in a mixed population of excess...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cell’s size, granularity, and target protein composition. It is used for a variety of applications such...electronics systems, FACS instruments have specialized components that can divert a subset of cells. During FACS...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...excitation and emission wavelengths, it is also important to know the brightness of individual fluorophores...antibody-conjugated labeling. Even though the two compounds are completely different in nature (eGFP being...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...through click chemistry. Although these multi-component systems can be complicated to implement, they ...Ulrichs, H., Gaska, I., & Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by ...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...fragments, allowing ordered assembly of multiple components into a destination vector. The first step in ...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...

  10. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

  12. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

  17. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...mammalian CMV promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus...

  18. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...and office areas provide multiple streams for composting and recycling in the event the object in question...

  19. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...
Showing: 421 - 440 of 662 results