We narrowed to 507 results for: otos

1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...

2. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ... (GEF-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock...

3. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find Our List of CRISPR...

4. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ... CRISPR 101: Homology Directed Repair CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines ...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ... one time. Weissman’s group has made standard protocols available and they are working to further optimize...

6. 3 Challenges in Plant Synthetic Biology

   Type

   Blog Post

   Published

   July 22, 2014, 5:46 p.m.

   ...better understand developmental, metabolic, and photosynthetic processes. Often the tools developed for plant...

7. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

8. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...observations about the issue, assessed potential protocol modifications, and ultimately decided on the changes...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...Affinity Tags for Protein Purification. Current Protocols in Protein Science 73: . https://doi.org/10.1002...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been recognized ...

11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...University has used Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel...

12. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ... CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license...

13. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T (2016) A proposal for validation of antibodies...

14. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ... the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School....

15. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M,...

16. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...locus-specific knock-in of FKBP12F36V. Detailed protocols describing the use of these plasmids are available...

17. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...which is necessary for effective STED), and be photostable over a broad pH range. The four biometric probes...

18. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...in a single reaction. For detailed Golden Gate protocols, complete with helpful tips and tricks, see The...

19. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...explains 1-2 potential pitfalls of the intended protocol, and offers alternative solutions to demonstrate...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...particular tool has gained widespread popularity – and notoriety – as a tool of choice in the field: dCas9 fused...