We narrowed to 631 results for: rel

...array of biological contexts.  Proximity labelling relies on the labelling of neighboring proteins within...interactions in vivo (Ge et al., 2019). This technique relies on an S. auerus transpeptidase sortase A variant...

...a fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence...on collateral cleavage by the enzyme and instead relies on its activity on the target regions thus making...

... lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version...measurement of different RTKs. Although this sensor relies on a change in localization to the cell membrane...

... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

... traditional restriction enzyme cloning we once relied exclusively on. In this blog post, I will go over...isn’t ideal for synthetic biology standards, which rely up on the re-use of parts between experiments. In...

...etc. This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help... try to keep conversations focused on research relevant objectives and their tangible resources. For example...

...to growth factors, some of the most clinically-relevant proteins are also the hardest to study. One common... improve more physiologically and endogenously relevant models, tools that increase confidence become ...

...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...and test multiple mice with the drug rather than rely on the results of a single mouse. Biological replicates...

...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

...epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed repair (HDR) to...they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of single guide...

...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...

...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...photons achievable with photoswitchable dyes. Correlative light and electron microscopy (CLEM) is an imaging...

...60 minutes of illumination, highlighting its reliability for long-term experiments (Salari et al., 2019...unconjugated dye molecules are critical for accurate and reliable imaging and assay results (Hayashi-Takanaka et...

...two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple target transcripts...Streptomyces Some of the most industrially and clinically relevant bacterial species reside in the genus Streptomyces...

...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year...

...-specific topic, allowing you to easily access relevant information for planning, executing, and troubleshooting...