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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 421 - 440 of 628 results

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...

  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...etc. This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help... try to keep conversations focused on research relevant objectives and their tangible resources. For example...

  7. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...and test multiple mice with the drug rather than rely on the results of a single mouse. Biological replicates...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

  12. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...debating how to fix this.  As scientists, we work tirelessly in the lab where the groundbreaking research ...training as an academic scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...

  14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged multiple...photons achievable with photoswitchable dyes. Correlative light and electron microscopy (CLEM) is an imaging...

  16. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year...

  19. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant drawback of working...
Showing: 421 - 440 of 628 results