We narrowed to 465 results for: try

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...

3. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...

5. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

   Type

   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....

6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...

7. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...

8. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...

10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...

11. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...

13. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

14. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

15. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...Next-Generation Sequencing Technologies. Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857...

16. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc...

17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina...

18. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral...