We narrowed to 460 results for: try
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TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...
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PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m...., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m.... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....such reagents as outlined by your institution or country. Have any other questions? Feel free to post them... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....going to graduate school, Joe taught high school chemistry for a year. The teaching/mentoring bug never went... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m.... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m.... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS. Thus, the ... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m.... into the desired repository. They also support entry of required metadata, with the option of adding ... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... Basics of Western Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies dG can... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina...