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We narrowed to 599 results for: REV;

Showing: 221 - 230 of 599 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...the problematically low fluorescent intensity of previous versions of Brainbow. Developed from Brainbow ...visual system, which has been difficult to study previously (Hadjieconomou et al., 2011). Cell tracing in...distances across multiple fields of view, whereas previous Brainbow technologies were useful for discriminating...

  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...efficiently by (1) improving editing accuracy by preventing re-editing and (2) optimizing “cut-to-mutation... was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target sequence once... mutation incorporation probabilities our data revealed, and found that for generating a homozygous HDR...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish... known as AAV2-retro) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...gathering data. Former lab members can give open, revealing information about the culture and lab head. While...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...record notes and observations so you have data to review when making a decision. If you don’t see a culture...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...biased and unbiased approaches. As suggested in a review by Tycko et al., 2016, unbiased sequencing and ...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature...specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID: 27087594...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

  8. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks...mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target...

  9. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

  10. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...
Showing: 221 - 230 of 599 results