We narrowed to 525 results for: ARL
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TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel...
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Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m.... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP, cyan... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....Indiana University School of Medicine. She is particularly interested in science communication and fundamental... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m.... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....www.protocol-online.org/ Protocol Online is one of the early protocol sharing websites. It was created in 1999... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs.... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....consistent dilution factors, change pipette tips regularly, and mix samples very well. Use duplicates or... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m...., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....adventure, just remember that even though these early steps don’t even involve antibodies, they are still...