We narrowed to 575 results for: BASE;
-
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....calcium indicator, OCaMP (Aggarwal et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). ...scaffold of OCaMP) are shaded in orange. Data from FPbase.org. Scientists have developed calcium indicators...
-
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....statement. Make certain to choose your recommenders based on your relationships to the person as well as their...resources, we created STEAMid, for a nationwide database of STEAM internships for high school, college... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m..... Such disparities might suggest underlying sex-based influences on physiologic or pathologic processes... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....reliable sequence data back for the first 50-100 base pairs of your sequencing read. You can expect to...mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection, although...help of DNA-editing software which integrates our database of over 2000 primers. Two 96-well PCR plates are...Finally, you will want to have a well-organized database of expected sequences, either for the inserts ...Get Plasmid Backbone Information from Our Vector Database ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously introduce a pair... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....with BioRender.com. To make channelrhodopsin-based optogenetics minimally-invasive, Frances Arnold’... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....distinguished. The UCLA Library offers some Powerpoint based templates with different column numbers for a good... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m...., Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks.... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m....difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....got a full time position with a Washington D.C.-based communications consulting firm, Palladian Partners... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12...