We narrowed to 556 results for: ESC;

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

2. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

3. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

4. Viral Vectors 101: Types of viruses

   Type

   Blog Post

   Published

   June 6, 2023, 1:15 p.m.

   ...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...

5. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

6. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...

7. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

8. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

9. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

10. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ... the exact nature of your mutations. A great description of the plethora of available sequencing options...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...worries, I've summarized the three strategies described in this post in the graphic below - including ...

13. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

15. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...candidate genes in TS iPSCs to identify whether any rescue cell migration and contraction defects. The lab...

17. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...to receive deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in...

18. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

19. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive...

20. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...Addgene’s blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...