We narrowed to 557 results for: ESC;

...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...

...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

... the exact nature of your mutations. A great description of the plethora of available sequencing options...

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...

...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

...worries, I've summarized the three strategies described in this post in the graphic below - including ...

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

... interactions in events that occur on a short timescale, such as during drug action or cell signaling....

...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein degradation...

...candidate genes in TS iPSCs to identify whether any rescue cell migration and contraction defects. The lab...

...to receive deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in...