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We narrowed to 867 results for: FER

Showing: 441 - 460 of 867 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... to determine the pros and cons of using many different types of gRNA design software. When you are happy...inserted or deleted. Each lab will use a slightly different method to genotype their pups. Taking a toe, a...weeks old and use the ear tag method (creating different patterns of ear holes and using that material ...latest and greatest in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target...plasmid into zygotes." Development, growth & differentiation 56.1 (2014): 122-129. PubMed PMID: 24372541...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...labs. While many labs approach the situation differently, some adopting simple note-taking tools, others...functionalities with experiment records. Many different types of ELNs are available on the market today... comparison to paper-based record keeping, ELNs offer functionalities that are hard to imagine on paper...desired functionalities i.e. main priorities of different ELN features, ranging from whether respondents... allows you to assign specific permissions to different lab members to avoid any issues later on. Do you...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...interviews, or get several interviews but no job offers, is a failure to clearly articulate one's talents...to convince me that their search committee is different and needs to be told all of the nuances of the...technologies, i.e. video interviews. For point of reference, I submitted my applications all by snail mail...hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with your search...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

  4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion from N- to C-terminus...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...advisable to generate multiple constructs with different insertions, since it is difficult to predict which...can follow him on twitter: @joachimgoedhart.   References Adjobo-Hermans, Merel JW, et al. "Real-time visualization...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...delivering scFvs that inhibit HIV.   References and Resources References: Ahmad ZA, Yeap SK, Ali AM, Ho WY...for high affinity scFvs. Bound phage are then proliferated by infecting bacteria for additional rounds ... high affinity binding. Bound phage are then proliferated by infecting bacteria for additional rounds ...science.3140379  Bradbury ARM, Sidhu S, Dübel S, McCafferty J (2011) Beyond natural antibodies: the power...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded guide RNA (gRNA...agrobacterium-mediated delivery. Summary While different in many of the specifics - promoters used, precise...mediated genome engineering in plants isn’t all that different from how it’s used in other systems. Luckily, ... don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov...of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... Even low-frequency editing could make a huge difference in DMD, and it’s estimated that exon skipping...respectively. Although each study used slightly different methods, they each observed the same positive ...disease type C. Although the three DMD studies referenced here represent a great step forward for CRISPR...many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness and...treatments may also be closer than we might expect.   References 1. Kaiser J. CRISPR helps heal mice with muscular...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...bottom of this post for a basic synopsis of the differences amongst the Brainbow systems. Pre-Brainbow techniques...to the promoter will be expressed. This system differs from Brainbow-1.0 in that transient Cre recombination...needed to mark individual neurons, the system also suffered from a number of limitations. First, the imaging...controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to ...2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial expression...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...PiggyBac transposons, the Woltjen Lab compared different variants of the polycistronic cassettes and discovered...a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...is a new member of this class that works via a different mechanism - the PhoCl protein is irreversibly ... are other RFP variants available that may be preferred for their intended uses, they provide a well-defined...of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown ...defined domains can be swapped onto other proteins to confer functionality. In Narayan et al., they used this...

  10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details on the assay, including...however, a physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a ...on the membranes will then be exposed to a lysis buffer to release recombinant proteins directly onto the...then suspend a freshly harvested dose of cells (preferably in log phase) in blocking solution (around 108...thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al. "Revisiting phage...

  11. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...“NCBI Site Preferences” box. Once on the Preferences page, navigate to “PubMed Preferences” and click ... that you know how to navigate to the PubMed Preferences and Filters pages, be sure to check out the other...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure... pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO hChR2(H134R...

  13. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...modified allele is selectable, it can be transferred to a different genetic background via P1 transduction...Figure 2). In the following sections, key steps that differ from traditional restriction enzyme cloning will...could be used to move the modification into a different background, if needed. This approach is best suited...science communication and in vitro diagnostics.     References 1. Costantino N, Court DL. Enhanced levels of...

  14. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition... Jennifer Tsang...

  15. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan degradation... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates kynurenine...M, Bhat M, Schuppe-Koistinen I, Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, ...

  16. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified ...the monkey story hit the presses, another very different bit of CRISPR news was circulating. Scientists... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed...

  17. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ... online. A few examples are provided here for reference: Untergasser Lab Preparation of chemically competent...use the Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells...then wash and resuspend the cells in the provided buffers. The competent cells are then aliquoted and stored...protocol)  Cells prepared using the Z-competent TM buffer can be easily transformed without a heat-shock ...

  18. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...finding the best way to organize your time. At a conference, you could be honing your science communications...that lab and interacting with the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator...Addgene blog Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find... Jennifer Tsang...

  19. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...shipped over 350,000 individual plasmids to 5,000 different research institutions. This has given us a unique...by the influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids...HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different ubiquitin plasmids in the repository. 100,000 ... and modified by so many labs and for so many different purposes that we had to create a CRISPR page to...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making an AAV1...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ...
Showing: 441 - 460 of 867 results