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We narrowed to 902 results for: Ide

Showing: 441 - 460 of 902 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com. Inosine...act on newly-deaminated inosines. Pro tip! The nucleoside inosine consists of the nucleobase hypoxanthine...transversion editor AYBEv3. This tool still retained considerable A → G editing activity at most sites, in addition...initial editor AXBE, for A → X base editor (X = any nucleotide). To create a more specific A → C base editor...be useful for saturation mutagenesis, and they provide a strong proof of concept for further development...efficiency, purity, and indel rate of each tool vary considerably. And there’s so much more exciting protein engineering...base editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook ...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...must be met. In the Department of Labor’s general guide to the effect on higher education, you can see that...and Brandeis universities have stated that they consider postdocs paid on stipends to be FLSA exempt, and...institutions; and that institutions which can more easily provide bridge funding to absorb the cost will encounter...jobs that the majority of postdocs end up getting outside academia. There is also a valuable lesson for the...level they will be raised to on Dec 1st - across a wide consensus of academic stakeholders. There was ample...going away, and it is becoming ever clearer that outside scrutiny of science and the academic system is ...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...invariably inconclusive and unreliable. Proper controls provide the constant variables that enable the correct ...functionality of your experimental system and help identify opportunities for troubleshooting or optimization...experiment, untreated cells should be included as these provide the baseline/standard against which other samples...correct interpretation of the results because it provides an important reference point when analyzing the... but the papers cited in the reference section provide more in depth information. Technical replicates... time consuming and ultimately more important. Ideally, each biological replicate should use fresh media...plasmids, be performed on different days, etc, but outside constraints may impose some limitations and you...

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... of multiple animals on different occasions via video, generating huge amounts of data. When published...experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...subsequently clarified what the policy intent was and provided an FAQ list to further explain the policy. The fact...variation is calculated and placed in the legend. Widely reported difficulties in reproducing experimental...the author or their host institution expected to provide data in formats exported to common file types? ...could be mitigated by on-line services such as SlideShare, Figshare and Dataverse. Just as Addgene helps...than just a single projection of it, could allow idea generation and extension, based on existing, published...

  5. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...with them. If you decided to make your own competent cells using the protocols provided above, then you ...cells are available online. A few examples are provided here for reference: Untergasser Lab Preparation...phase, then wash and resuspend the cells in the provided buffers. The competent cells are then aliquoted...Stbl3TM. How to transform your plasmid DNA If you decided to use commercial competent cells, then you're ...transforming your cells? Check out our transformation guide.   ...

  6. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...mutations found in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more...mouse line. Over the years the fly has become an ideal model organism to study an array of topics including... to create transgenic animals. Zebrafish are an ideal model organisms for embryonic development studies... genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes. C. elegans...quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling." Proceedings of ...Menezes, Regina, et al. "From the baker to the bedside: yeast models of Parkinson's disease." Microbial...Additional resources on the Addgene blog Read our quick guide to Drosophila Download the fluorescent proteins ...

  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...vectors (here and here).  Figure 3: JEDI-2P was identified from a high-throughput screen, selected for increased...existing sensors. Using genome mining and structure-guided engineering of potassium binding proteins, they...point for further engineering. Figure 5: Structure-guided design and genome mining both led to new sensors...Fluorescent Potassium Indicators through Structure-Guided and Genome Mining Strategies. ACS Sens. 2022 May...protein plasmids and resources Fluorescent protein guide: Biosensors Collection ...

  8. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...loading control: the intensity of each POI band is divided by the intensity of the loading control in the ...problems for proteins of very different abundance, consider the following example gel. Figure 5: An...intensity axis. But the loading control points are outside of the linear detection range. As a result, they... problematic for computational quantification. Outside the linear detection range, band intensity measurements...experimental conditions. Otherwise, you might want to consider a different loading control. Assumption #2: The...final blot. Quantifying the entire lane of protein provides a loading control that can be used for normalization...The necessity of and strategies for improving confidence in the accuracy of western blots. Expert Review...

  9. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping...before you even receive the product? How can we consider the environmental impact of those processes? Who...might sound risky in terms of contamination, but consider flow cytometers. These fluidic machines handle...through an EPA-approved process and no longer considered hazardous. Then, they use this material to produce...plastic lumber and distribute it to industrial and residential markets. Rethinking our approach to lab waste...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...Accessibility Guidelines (WCAG). These changes mean our content is both available and accessible to a wider community... Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs enabled by shared resources...part of the story. This year, we made significant strides in making science more accessible and reliable....community. As we enter 2025, your feedback continues to guide our innovation.  In response to a demand for more...rapidly, and with it, the needs of researchers worldwide. Through our growing community on Bluesky, our...

  11. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that ...apoptosis. For each of these 17 pathways, they identified a set of one to three mutant complementary DNAs... Wood says. “We want to make these available as widely as possible.” The researchers have plans to expand...References 1. Martz, Colin A., et al. "Systematic identification of signaling pathways with potential to confer...Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...How to put the wider scientific community at the service of COVID-19 research? We decided to give it a ...internationally COVID-19 Resources Canada is a database that provides information about reagents, funding and volunteer... the information is applicable to researchers worldwide. “COVID-19 has spread incredibly fast and without...can advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion...Read about COVID-19 research on the blog Visit our guide for external collaborations Resources on Addgene.org...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...proteoglycan (HSPG)3 AAV receptor (AAVR)2 Epidermal growth factor receptor (EGFR)3 AAV8 Laminin...there are up to thirteen natural serotypes of AAV identified so far, numbered AAV1, AAV2, and so on. In addition... the second number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure...AAVrg has not been thoroughly studied in contexts outside the nervous system to confirm its cellular receptors...addition to the toolbox, with the first paper identifying a binding receptor published this year (Dhungel...2008). Adeno-Associated Virus (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1,...Systemic Capsids Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request Browse all ...

  14. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...middle of the targeted region, as shown below.  To identify optimal nickase designs, IDT scientists Mollie...insert an EcoRI site using a single-stranded oligonucleotide (ssODN) donor. Using a D10A nickase to insert...nickase outperformed both WT Cas9 sites tested by a wide margin, exhibiting >20% repair efficiency. Clearly... is especially useful if there are no available guides close to the desired mutation site. Quick tips...small insertions or tags (ssODN; IDT Ultramer® Oligonucleotides) Use 100 nt homology arms for large insertions... Find CRISPR Nickase Plasmids Browse the CRISPR Guide ...

  15. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...every project we undertake and every service we provide is tested against fulfilling our mission. “Addgene...continuously improve all the extra services we provide like quality control, curation, technical service...starting new initiatives as opposed to fundraising.   Aside from enabling us to make all decisions with the ... keeping excellent records and maintaining confidentiality where it is appropriate. This grows our relationship...Addgene increases productivity by providing world-wide access to curated, high-quality data and reagents...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

  16. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...an introduction to plasmids. Plasmids 101 will provide you with an overview of general molecular biology.... Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less time researching... such as yeast and plants. In nature, plasmids provide one or more functional benefits to the host such... biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types...scientists can track their location or quantity inside a cell Plasmids 101: Green Fluorescent Protein...references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and troubleshooting...

  17. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...but overwhelmed; I had no idea where to start. Thankfully, my managers set aside time for me to complete...the lab! I got to assist production on a protocol video. Don't worry - no pipettes were harmed in the making...allowed me to succeed. As a team, we talked through slide design, discussion items, big takeaways, and timing...depositor welcome kits, note taking for protocol video production, and keeping inventory of our merchandise...talk to. This six month experience made me more confident in myself, my abilities, and my future - something...

  18. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ... at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   Resources Resources... a master? Here are a few questions to help you decide if a master’s might be for you. 1. Are you interested...path one you could see yourself taking? If so, consider setting up an informational interview with them...your interests and career goals. You might still decide to earn a PhD, but if I had to do grad school over...eBooks from Addgene, including the Science Career Guide\  ...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...smaller gel apparatus until I settled on using glass slides. With just the right amount of agarose, I could...could pour a very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour ...plastic and solder this thing together.  One of the downsides—there was no cover so you had to be careful not...

  20. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues ...respectively. mNeonGreen can be used as a fusion tag for a wide range of applications from traditional imaging to...resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent proteins and therefore...The team that developed mTFP1 further sought to identify why this protein was blue-shifted compared to ...blue-shifting more than others. During this process, they identified mWasabi, a bright, monomeric green fluorescent...variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and ...
Showing: 441 - 460 of 902 results