We narrowed to 461 results for: MEA;

1. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...resistance marker from the parental plasmid provides a mean for selecting for transformants which have taken...

2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ... the percentage of cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell...

3. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...activity or gene expression. Experimental output is measured and quantified. Created with BioRender.com. ...

4. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   Published

   Oct. 2, 2018, 12:56 p.m.

   ...failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders...

5. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...will cleave DNA frequently enough to result in a smear of fragments that get smaller in size as a function...

6. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...citizen, I was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia...

7. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   Published

   Nov. 6, 2015, 3:30 p.m.

   ...should not be confined by birth country or financial means. Instead, talent should be realized through equal...

8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ...plasmid amplification (Fischer, KB. 2019). This means that all DIO/FLEx AAV vectors will have some level...

9. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...achieve exponential amplification. What does fidelity mean for a ligase? DNA ligases generally prefer fully...

10. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ... all team members understand what roles they’re meant to fill and what their responsibilities are. That...

11. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...Modified from Aschauer et al. (2013) What does this mean to us? Well, an AAV user must determine which serotype...

12. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...the substrate with the target DNA sequence. This means cloning occurs in vivo, as compared to restriction...

13. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ...and your development. Taken together, all of this means that you need to introduce yourself to and talk ...

14. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...as their selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of...

15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...experiment and test them in parallel so you have a good measure of ICC assay specificity. We generally include ...

16. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...environments. These studies provide quantitative measurements of cellular motility, interactions, and response...

17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...while the efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous...

18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ... makes choosing the right tool confusing and it means that there is no one-size-fits-all approach. You...

19. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ...the construct is from our 'Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically...

20. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...important because ABEs have generally been slow, meaning that the Cas9 domain often lets go of the DNA before...