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Showing: 441 - 460 of 699 results

  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...peptides sequences into these variable loops is one method for changing a capsid’s tropism.  cap also encodes...the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog...

  3. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...

  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   Interested in...

  6. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2)   Read science...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...single particle creates many copies of itself, sometimes up to millions of new copies, which are released...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Isotype matters when constraining using the indirect method since a secondary antibody reactive to a certain... not be used for multiplexed indirect staining methods since the secondary antibody used will react to...

  9. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed...

  13. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...(2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat... of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276...

  14. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...communication itself, not the communication method, that determines if something is scientific or science communication...

  15. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright...

  16. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang...

  17. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...sensitive to sunlight and prone to developing metastatic melanomas and other cancers. Joshua Wang (bottom...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012...

  18. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship ...heavy price tag, especially if you're working on something other than software.  If you are a graduate student...

  19. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...description of the lab’s iTOP protein-only transduction method and its potential applications to CRISPR/Cas9 genome...osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies showed that...

  20. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...is because the host lab or institute provides something that you can’t obtain at your current institute...notes, and ask questions if you don’t understand something. Offer to help out wherever you can and don’t ...
Showing: 441 - 460 of 699 results