We narrowed to 711 results for: Met
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TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m.... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...new x-ray technique provides scientist with a new method to explore the inner structures of delicate plants...
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Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m.... in five years and hopefully I'll have worked something out!” We will get back to you on that, Tom! Connie...”. He also cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m.... for monitoring norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592...Making protein folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and eat, and...vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....fingerprint can be detected. Bourdeau et al calls this method acoustic collapse. Importantly, acoustic collapse...have already developed a high-throughput screening method for serial acoustic collapse to aid in the development... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....the figures of interest along with the article metadata, legend and user created notes on a “Pinterest...Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure.... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....peptides sequences into these variable loops is one method for changing a capsid’s tropism. cap also encodes...the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org... -
Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....scientists edit the same document may sound like something out of your nightmares, shared ownership of materials...communication within an existing collaboration, or start something new! Additional resources on the Addgene blog... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know in the ...Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering Interested in... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is...Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....ask and answer questions and hopefully discover something new. Science involves success and, more often,...not just the details of your science but also something about how science really works. 2) Read science... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....(2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat... of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb.2019.276... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...people) -> How can you make a decision about something like this without sufficient data? Starting to... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase...to decrease error rate, please use one of these methods to ensure your insert retains the 3' A overhang... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m...., peer-review is a touchstone of the scientific method, ensuring that published work meets the accepted...manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....limited expression levels of XFPs in the original method, we used the Tetracycline trans-activator system...Improved tools for the Brainbow toolbox." Nature methods 10.6 (2013): 540. PubMed PMID: 23817127. PubMed... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....communication itself, not the communication method, that determines if something is scientific or science communication... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... instead, which can’t be done if your selection method kills the transgene free individual. For example...studying large samples. RUBY Red But recently, something new has been added to the palette. RUBY is a bright...