Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 850 results for: NTS

Showing: 441 - 460 of 850 results

  1. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...understand how to make new treatments and technologies accessible to patients. And what better place to... through engagement with your local or state governments. Most communities have opportunities to join ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...policy fellowships in federal, state, and local governments, professional societies, industry, and beyond...

  2. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ... and what makes it difficult for many graduate students. As part of this discussion, we mention graphs...school. This is, of course, not real data, but many students discuss the third year dip (see graph). Niroshi...about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to the grad school process ...'s favorite advice to give to younger graduate students   Check Out Angela Depace's Talk on "Precision...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...key to its efficient use in screening experiments; large amounts of virus are required to ensure that ...plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the... We thank our many depositors for making these reagents available to the research community through Addgene...

  4. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the... provide bacteria protection against infectious agents. Several enzymes behind this immunity have already...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

  5. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    Published
    July 26, 2018, 12:46 p.m.
    ...together. Second, KTRs are easy to design. The key determinants of KTR function are the positions of the phosphorylatable...measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus...another variable to consider when designing KTR experiments. Third, KTRs are not well suited for monitoring... Regot, S., & Covert, M.W. (2018). Live-cell measurements of kinase activity in single cells using translocation...

  6. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...able to talk and own these accomplishments. Employers and academic departments want to be able to tell that...Leadership and management Take a lead from the amazing students at Washington University who started the BALSA...like FierceBiotech to “learn the business” Attend events with local networking and trade organizations ...

  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...used these knockout mice to show exactly which components of the known pathway that were essential for ... rapidly performed by targeting all potential components of a signaling pathway. For example, one could...PD-L1 on a cancer cell). In this screen, known components of the cytokine signaling pathway could be targeted...target surface proteins to determine which contact points are involved in a studied cellular behavior. This...accelerated immensely when we share knowledge and reagents. I have not intentionally excluded any libraries...

  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...following the trend and encouraging departments to showcase the work of students and staff by holding scientific...method to explore the inner structures of delicate plants and other organisms without destroying the original...organisms on your lab bench? Let us know in the comments.    Additional Resources on the Addgene Blog ...

  9. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ... is a database that provides information about reagents, funding and volunteer opportunities, protocols... hopefully help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque...including datasets and platforms for discussing new developments, and more. As researchers around the world continue...community work together to share open protocols, reagents, and other initiatives for #COVID19 research. ...

  10. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...peak absorption wavelengths and light intensity requirements of different opsins, studies have shown that...generates periodic motor commands for rhythmic movements like spontaneous swimming. To do so, the lab expressed... behaviors, the researchers measured the tail movements of 5 day old fish. The larvae were immobilized... groups of neurons that elicited similar tail movements, they were able to narrow down the possible specific...

  11. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...and hide individual vectors (shapes defined by 2D points connected by lines and curves) that you’ve drawn...Layers are useful for drawing figures with lots of elements. It took some playing around to figure out all...images to the Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces...resource not mentioned? Tell us about it in the comments section!  Additional resources on the Addgene...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...forms of antibodies and had begun developing Fab fragments and scFvs. But the potential for an even smaller... they compare to other antibodies and affinity reagents. But for a quick refresher, some of the advantages...target concave epitopes Suitability for in vivo experiments, due to their simple structure    sdAbs in research... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

  13. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required for... and modifying various extinct transposases. Improvements have been made since then by increasing the ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

  14. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Bozeman Sunday night, it was easy to spot meeting participants. They were the ones holding poster tubes (or...Zhang's introduction. His willingness to share reagents so freely with the academic community has clearly...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...who contact us and want a guarantee that their experiments will work (yes, this happens): Science is hard...

  15. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit their growth. Although...be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high concentrations... the mutation. When designing these types of experiments it is best practice not to use the same resistance...Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find Antibiotic Resistance ...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    .... In neutral environments, dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless...mechanism as Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences...18094608. Sargsyan, A., et al. "Rapid parallel measurements of macroautophagy and mitophagy in mammalian...

  17. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...origin of replication (ori) and all of its control elements. The ori is the place where DNA replication begins...lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind that plasmids... medium copy numbers can still express massive amounts of protein given the proper promoter and growth...something clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, ...

  18. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...error rates compared to other RTs. MarathonRT represents a major step forward in facilitating techniques...understanding thereof have the potential to provide improvements to existing technologies based on polymerase...segment Nanobodies are single domain antibody fragments typically engineered from heavy-chain only antibodies... C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the...

  19. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...if we can help them share and distribute other reagents. Virus distribution came up time and time again...this frustration and getting researchers to the experiments they’re interested in faster by producing, titering...you out. Of course, lentiviruses are used in experiments other than just those involving CRISPR and so...first two can serve as controls in a variety of experiments, while rtTA can be used to activate expression...

  20. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...mammalian cells, likely due to high temperature requirements. To solve this problem, Gao et al. searched ...potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of the DNA...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...
Showing: 441 - 460 of 850 results