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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 753 results for: Nes

Showing: 441 - 460 of 753 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...options: Early detection, immunotherapy, and angiogenesis inhibitors New research is also going into two...bevacizumab (Avastin). This drug (known as an angiogenesis inhibitor) prevents the growth of new blood ...asbestos. Nonprofits like the Asbestos Disease Awareness Organization (ADAO) are working toward such a ...

  2. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...sharing information through the use of popular Chinese social media platforms.  As you can imagine, shipping...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  3. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Austrian physician, Andreas Rett, who tried to raise awareness and published his observations in a German medical...global transcriptional regulator of thousands of genes. Studies have suggested roles in transcriptional..., Zoghbi HY (2013) An AT-Hook Domain in MeCP2 Determines the Clinical Course of Rett Syndrome and Related...

  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...(Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous cleavage and degradation of neighboring...ability to cleave non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform...Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...my days as an undergrad working with cancer cell lines. As I started planning out my own experiments in...when prioritizing, you should always be aware of deadlines and think about what they mean in terms of the...favorite podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination recommends testing...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier...2P has faster response kinetics and increased brightness, sensitivity, and photostability under two-photon...screen, selected for increased voltage response, brightness, and photostability. It enables sustained deep-tissue...

  7. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...signal amplification. Another reason is cost effectiveness. Generating and validating primary antibodies...outcome of your assay. So how do you choose good ones? Choosing a secondary is often much more straightforward...non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody will ...

  8. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...efficient in human cells on average, but its effectiveness varied depending on the chromosomal location...cell type. Multiple labs worked to improve its effectiveness through directed evolution, introducing mutations...Kleinstiver’s lab engineered new variants of S. pyogenes Cas9. While the wild-type enzyme requires a PAM...

  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms which...several aspects of editing. First, they devised guidelines for designing single-stranded repair templates...on gene regulation in nematode molting and spermatogenesis. His group also builds tools to improve genome...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...are amenable to genetic manipulation, nor can all genes be easily knocked down or out.   Example: You just...data that convinced you of your antibody’s trustworthiness. You may also consider sharing your validation..., Anderson AP, Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...transition Base changes between purines (A ↔ G) or between pyrimidines (C ↔ T).   Base transversion  ...conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...above 30% for a variety of targets in human cell lines, with an average indel frequency of only 1.1%. These...which is significantly more effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following... of Tad-CBEs that can precisely target single cytosines in the base editing window, reducing bystander...

  13. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...Created by Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at...at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford...

  14. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...blog posts showcasing the work of depositors) Wednesday: Why deposit? Thursday: Throwback Thursday (Blue...

  15. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...AAV9 is so strong that you can even see dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording..., when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  We love negative...

  18. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can ...of published and commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include...

  19. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...library (~60,000 gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs...APOBEC1 causes genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE ...

  20. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...biggest changes, there are plenty of less obvious ones to discover as you browse. You may find one of your...choices informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check...
Showing: 441 - 460 of 753 results