We narrowed to 517 results for: PROC

1. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ... a novel gene, is not always a straightforward process. Rather than try to directly detect your gene of...

2. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...no PCR, no gel purification; the whole cloning process is so simplistic once you have your parts. That...

3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...that need to be considered during the planning process. For CRISPR experiments, the following framework...

4. Hot Plasmids: Winter 2025

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   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ... inserted. B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY ...

5. Pooled Library Amplifications

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   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ... reduce recombination during the amplification process. In liquid culture, all of the bacteria can grow...

6. Antibodies 101: Flow Compensation

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   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...

7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...types makes them useful models for developmental processes and promising therapeutic tools. The two unique...

8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...indeed possible, with correct, in-frame assembly proceeding in 99% of 12-fragment assemblies and over 90%...

9. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...could be used to study the role of aaRSs in these processes. To create the antibody toolbox, they screened...

10. 10 Basic tips for mammalian cell culture

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    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. ...

11. 3D Printing Meets CRISPR Cas9

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    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...protein. At some point during this interrogation process, they will almost invariably say something like...

12. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...from rogue cells when something does go awry. The process of programmed cell death, or apoptosis, is one ...

13. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ... comfortable terms helped guide me through the process. As I mentioned, my graduate office staff was amazingly...

14. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...MMEJ doesn’t get a lot of press. However, this process accounts for a percentage of the mutations seen...

15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... enabling the study of dynamic, physiological processes. Re-engineering GFP to increase its range of color...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ... to genome editing of mouse zygotes, through a process known as CRISPR-EZ (CRISPR RNP Electroporation ...

17. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ... of a specific genomic region was an intensive process. One method required insertion of a recognition...

18. Getting to Know Expansion Microscopy

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    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ....)  Step 5: Image   Figure 1: The ExM process allows you to physically magnify a sample so you...

19. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...

20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...consequence of the undermining of the scientific process and the marginalization of science, particularly...