We narrowed to 479 results for: RET

1. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in...

2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

   Type

   Blog Post

   Published

   July 10, 2018, 1:08 p.m.

   ...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching...

3. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...

4. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ...drawback for therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed...

5. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...promise it isn’t.) The intuitive, qualitative interpretation of this gel is misleading. We can see why on...

6. Antibodies 101: Beyond Surface Labeling

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   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...

7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...by Wrenbeck et al but it’s likely that longer stretches of sequence could be used. However, a practical...

8. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...

9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...color for its entire lifetime and daughter cells retain the same color, allowing for the fate mapping of...

10. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...Spinach, a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI...

11. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...). E. coli as a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net...

12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...of protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013):...

13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma...

14. Thoughts on the Future of Research 2015 Boston Symposium

    Type

    Blog Post

    Published

    Nov. 25, 2015, 4 p.m.

    ...academic research. Panelist Moon Duchin provided a concrete example of how underrepresented individuals are...

15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...and -2. The general structure of Brainbow-1.0 is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2...

16. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...notebooks also play a role here by making it easier to retain searchable, thorough notes on how lab procedures...

17. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ... effective, so long as care is taken in the interpretation of results by requiring multiple sequences ...

18. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1...

19. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    Published

    June 27, 2023, 1:15 p.m.

    ...proteins responsive to light in combination with retinal) variations designed to give researchers flexibility...

20. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ... patterns with several overlapping targets to interpret. Check out (Jambor et al., 2021) for more guidelines...