We narrowed to 464 results for: SHI

1. Five Popular Model Organisms

   Type

   Blog Post

   Published

   April 11, 2019, 12:39 p.m.

   ... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):...

2. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...

3. CRISPR 101: Homology Directed Repair

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   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...double-strand break repair: how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed...

4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   Blog Post

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   May 5, 2020, 1:15 p.m.

   ...cellular aspects of CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested...

5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   Published

   Jan. 16, 2025, 3:42 p.m.

   ...N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....

6. How to Design Your gRNA for CRISPR Genome Editing

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   Blog Post

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   Sept. 24, 2020, 1:15 p.m.

   ...often leads to the generation of indels and thus frameshifts that disrupt the protein-coding capacity of a...

7. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K...

8. CRISPR 101: Off-Target Effects

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   Blog Post

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   Feb. 8, 2024, 2:15 p.m.

   ...double strand break (DSB), the event required for frameshift and knock-in mutations. If one nickase acts on...

9. PiggyBac-ing Through the Genome Editing Field

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   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent...

10. Finding Your Perfect Job After University

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    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...would find a genetics related role soon after finishing my MSc. The masters program was 6 months of classes...

11. Important Considerations When Using AAVs

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    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...gene delivery in an efficient and non-pathogenic fashion. Work improving AAV tissue tropism, transduction...

12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ..., which I’d say is pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting...

13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...manager at genOway.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

14. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

    Published

    March 7, 2023, 2:15 p.m.

    ... of comparisons. The Bonferroni does this by establishing a significance threshold at a p value less than...

15. A History of Genome Engineering in Popular Culture

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    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...were space and robots. Warping to stars in space ships and the rise of killer machines that exterminate...

16. Sequencing Options for CRISPR Genotyping

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    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying...

17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...appropriate containers. The machine is useful for distinguishing cell populations that have been tagged with...

18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...primers flanking the tag sequence and expect a size-shift in the PCR product for knock-in lines. Troubleshooting...

19. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...plasmids from this paper at Addgene. 3. Sakuma, Tetsushi, et al. “Multiplex genome engineering in human...

20. Antibody Validation for Flow Cytometry

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    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...