We narrowed to 460 results for: SHI

1. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 3:42 p.m.

   ...N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....

2. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...double strand break (DSB), the event required for frameshift and knock-in mutations. If one nickase acts on...

3. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K...

4. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...often leads to the generation of indels and thus frameshifts that disrupt the protein-coding capacity of a...

5. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent...

6. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...would find a genetics related role soon after finishing my MSc. The masters program was 6 months of classes...

7. Deep Dive: Statistical Tests (Comparisons)

   Type

   Blog Post

   Published

   March 7, 2023, 2:15 p.m.

   ... of comparisons. The Bonferroni does this by establishing a significance threshold at a p value less than...

8. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   Published

   Feb. 25, 2020, 2:15 p.m.

   ...were space and robots. Warping to stars in space ships and the rise of killer machines that exterminate...

9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

   Type

   Blog Post

   Published

   Oct. 17, 2019, 12:28 p.m.

   ...manager at genOway.     Acknowledgement:Through a partnership with genOway, we are able to distribute materials...

10. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying...

11. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...gene delivery in an efficient and non-pathogenic fashion. Work improving AAV tissue tropism, transduction...

12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ..., which I’d say is pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting...

13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...primers flanking the tag sequence and expect a size-shift in the PCR product for knock-in lines. Troubleshooting...

14. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...appropriate containers. The machine is useful for distinguishing cell populations that have been tagged with...

15. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...plasmids from this paper at Addgene. 3. Sakuma, Tetsushi, et al. “Multiplex genome engineering in human...

16. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...

17. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ...software. This category is important for users wishing to select tools based on their comfort level with...

18. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...

19. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer CT. The MIQE guidelines...

20. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...